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Published 13 March 2006. doi:10.1083/jcb.200511038
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 172, Number 6, 875-884
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Article

 The Drosophila melanogaster Cajal body

Ji-Long Liu1, Christine Murphy1, Michael Buszczak1, Sarah Clatterbuck2, Robyn Goodman2, and Joseph G. Gall1

1 Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21218
2 Department of Biology, Johns Hopkins University, Baltimore, MD 21218

Correspondence to Joseph G. Gall: gall{at}ciwemb.edu

Cajal bodies (CBs) are nuclear organelles that are usually identified by the marker protein p80-coilin. Because no orthologue of coilin is known in Drosophila melanogaster, we identified D. melanogaster CBs using probes for other components that are relatively diagnostic for CBs in vertebrate cells. U85 small CB–specific RNA, U2 small nuclear RNA, the survival of motor neurons protein, and fibrillarin occur together in a nuclear body that is closely associated with the nucleolus. Based on its similarity to CBs in other organisms, we refer to this structure as the D. melanogaster CB. Surprisingly, the D. melanogaster U7 small nuclear RNP resides in a separate nuclear body, which we call the histone locus body (HLB). The HLB is invariably colocalized with the histone gene locus. Thus, canonical CB components are distributed into at least two nuclear bodies in D. melanogaster. The identification of these nuclear bodies now permits a broad range of questions to be asked about CB structure and function in a genetically tractable organism.

Abbreviations used in this paper: CB, Cajal body; dSMN, Drosophila melanogaster SMN protein; GV, germinal vesicle; HLB, histone locus body; scaRNA, small CB-specific RNA; SMN, survival of motor neurons; snRNA, small nuclear RNA; snoRNA, small nucleolar RNA; UAS, upstream activating sequence.


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