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Published 27 March 2006. doi:10.1083/jcb.200508116
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 172, Number 7, 991-998
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 A role for the RabA4b effector protein PI-4Kß1 in polarized expansion of root hair cells in Arabidopsis thaliana

Mary L. Preuss1, Aaron J. Schmitz2, Julie M. Thole1,3, Heather K.S. Bonner1, Marisa S. Otegui4, and Erik Nielsen1,3

1 Donald Danforth Plant Science Center, St. Louis, MO 63132
2 Department of Plant Biology, Michigan State University, East Lansing, MI 48824
3 Department of Biology, Washington University in St. Louis, St. Louis, MO 63130
4 Department of Botany, University of Wisconsin, Madison, WI 53706

Correspondence to Erik Nielsen: enielsen{at}danforthcenter.org


Abstract
The RabA4b GTPase labels a novel, trans-Golgi network compartment displaying a developmentally regulated polar distribution in growing Arabidopsis thaliana root hair cells. GTP bound RabA4b selectively recruits the plant phosphatidylinositol 4-OH kinase, PI-4Kß1, but not members of other PI-4K families. PI-4Kß1 colocalizes with RabA4b on tip-localized membranes in growing root hairs, and mutant plants in which both the PI-4Kß1 and -4Kß2 genes are disrupted display aberrant root hair morphologies. PI-4Kß1 interacts with RabA4b through a novel homology domain, specific to eukaryotic type IIIß PI-4Ks, and PI-4Kß1 also interacts with a Ca2+ sensor, AtCBL1, through its NH2 terminus. We propose that RabA4b recruitment of PI-4Kß1 results in Ca2+-dependent generation of PI-4P on this compartment, providing a link between Ca2+ and PI-4,5P2–dependent signals during the polarized secretion of cell wall components in tip-growing root hair cells.

M.L. Preuss and A.J. Schmitz contributed equally to this paper.

Abbreviations used in this paper: AtCBL, Arabidopsis thaliana calcineurin B–like proteins; LKU, lipid kinase unique; NH, novel homology; T-DNA, transfer DNA; WT, wild type.


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