Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 2537K) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dantuma, N. P. Articles by Neefjes, J. Search for Related Content PubMed PubMed Citation Articles by Dantuma, N. P. Articles by Neefjes, J. Pubmed/NCBI databases Substance via MeSH Related Collections Related Article Social Bookmarking                            What's this?

A dynamic ubiquitin equilibrium couples proteasomal activity to chromatin remodeling

¹ Department of Cell and Molecular Biology, The Medical Nobel Institute, Karolinska Institutet, S-171 77 Stockholm, Sweden
² Division of Tumor Biology, The Netherlands Cancer Institute, 1066 CX, Amsterdam, Netherlands

Correspondence to Nico P. Dantuma: nico.dantuma{at}ki.se; or Jacques Neefjes: j.neefjes{at}nki.nl

Protein degradation, chromatin remodeling, and membrane trafficking are critically regulated by ubiquitylation. The presence of several coexisting ubiquitin-dependent processes, each of crucial importance to the cell, is remarkable. This brings up questions on how the usage of this versatile regulator is negotiated between the different cellular processes. During proteotoxic stress, the accumulation of ubiquitylated substrates coincides with the depletion of ubiquitylated histone H2A and chromatin remodeling. We show that this redistribution of ubiquitin during proteotoxic stress is a direct consequence of competition for the limited pool of free ubiquitin. Thus, the ubiquitin cycle couples various ubiquitin-dependent processes because of a rate-limiting pool of free ubiquitin. We propose that this ubiquitin equilibrium may allow cells to sense proteotoxic stress in a genome-wide fashion.

Abbreviations used in this paper: GFP-Ub, GFP-ubiquitin; PAGFP, photoactivatable GFP; uH2A, ubiquitylated histone H2A.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

Related Article

Ubiquitin tug-O-war

Rabiya S. Tuma
J. Cell Biol. 2006 173: 3. [Full Text] [PDF]

This article has been cited by other articles:

• Marteijn, J. A., Bekker-Jensen, S., Mailand, N., Lans, H., Schwertman, P., Gourdin, A. M., Dantuma, N. P., Lukas, J., Vermeulen, W. (2009). Nucleotide excision repair-induced H2A ubiquitination is dependent on MDC1 and RNF8 and reveals a universal DNA damage response. JCB 186: 835-847 [Abstract] [Full Text]  
• Raspe, M., Gillis, J., Krol, H., Krom, S., Bosch, K., van Veen, H., Reits, E. (2009). Mimicking proteasomal release of polyglutamine peptides initiates aggregation and toxicity. J. Cell Sci. 122: 3262-3271 [Abstract] [Full Text]  
• Dantuma, N. P., Lindsten, K. (2009). Stressing the ubiquitin/proteasome system. Cardiovasc Res 0: cvp255v2-cvp255 [Abstract] [Full Text]  
• Pang, B., Neijssen, J., Qiao, X., Janssen, L., Janssen, H., Lippuner, C., Neefjes, J. (2009). Direct Antigen Presentation and Gap Junction Mediated Cross-Presentation during Apoptosis. J. Immunol. 183: 1083-1090 [Abstract] [Full Text]  
• Grou, C. P., Carvalho, A. F., Pinto, M. P., Huybrechts, S. J., Sa-Miranda, C., Fransen, M., Azevedo, J. E. (2009). Properties of the Ubiquitin-Pex5p Thiol Ester Conjugate. J. Biol. Chem. 284: 10504-10513 [Abstract] [Full Text]  
• Salomons, F. A., Menendez-Benito, V., Bottcher, C., McCray, B. A., Taylor, J. P., Dantuma, N. P. (2009). Selective Accumulation of Aggregation-Prone Proteasome Substrates in Response to Proteotoxic Stress. Mol. Cell. Biol. 29: 1774-1785 [Abstract] [Full Text]  
• Shao, G., Lilli, D. R., Patterson-Fortin, J., Coleman, K. A., Morrissey, D. E., Greenberg, R. A. (2009). The Rap80-BRCC36 de-ubiquitinating enzyme complex antagonizes RNF8-Ubc13-dependent ubiquitination events at DNA double strand breaks. Proc. Natl. Acad. Sci. USA 106: 3166-3171 [Abstract] [Full Text]  
• Dayal, S., Sparks, A., Jacob, J., Allende-Vega, N., Lane, D. P., Saville, M. K. (2009). Suppression of the Deubiquitinating Enzyme USP5 Causes the Accumulation of Unanchored Polyubiquitin and the Activation of p53. J. Biol. Chem. 284: 5030-5041 [Abstract] [Full Text]  
• Carrettiero, D. C., Hernandez, I., Neveu, P., Papagiannakopoulos, T., Kosik, K. S. (2009). The Cochaperone BAG2 Sweeps Paired Helical Filament- Insoluble Tau from the Microtubule. J. Neurosci. 29: 2151-2161 [Abstract] [Full Text]  
• Wang, Q., Mora-Jensen, H., Weniger, M. A., Perez-Galan, P., Wolford, C., Hai, T., Ron, D., Chen, W., Trenkle, W., Wiestner, A., Ye, Y. (2009). ERAD inhibitors integrate ER stress with an epigenetic mechanism to activate BH3-only protein NOXA in cancer cells. Proc. Natl. Acad. Sci. USA 106: 2200-2205 [Abstract] [Full Text]  
• Alekseev, S., Luijsterburg, M. S., Pines, A., Geverts, B., Mari, P.-O., Giglia-Mari, G., Lans, H., Houtsmuller, A. B., Mullenders, L. H. F., Hoeijmakers, J. H. J., Vermeulen, W. (2008). Cellular Concentrations of DDB2 Regulate Dynamic Binding of DDB1 at UV-Induced DNA Damage. Mol. Cell. Biol. 28: 7402-7413 [Abstract] [Full Text]  
• Sabbioneda, S., Gourdin, A. M., Green, C. M., Zotter, A., Giglia-Mari, G., Houtsmuller, A., Vermeulen, W., Lehmann, A. R. (2008). Effect of Proliferating Cell Nuclear Antigen Ubiquitination and Chromatin Structure on the Dynamic Properties of the Y-family DNA Polymerases. Mol. Biol. Cell 19: 5193-5202 [Abstract] [Full Text]  
• Ren, J., Pashkova, N., Winistorfer, S., Piper, R. C. (2008). DOA1/UFD3 Plays a Role in Sorting Ubiquitinated Membrane Proteins into Multivesicular Bodies. J. Biol. Chem. 283: 21599-21611 [Abstract] [Full Text]  
• Mayor, T., Graumann, J., Bryan, J., MacCoss, M. J., Deshaies, R. J. (2007). Quantitative Profiling of Ubiquitylated Proteins Reveals Proteasome Substrates and the Substrate Repertoire Influenced by the Rpn10 Receptor Pathway. Mol. Cell. Proteomics 6: 1885-1895 [Abstract] [Full Text]  
• Murakawa, Y., Sonoda, E., Barber, L. J., Zeng, W., Yokomori, K., Kimura, H., Niimi, A., Lehmann, A., Zhao, G. Y., Hochegger, H., Boulton, S. J., Takeda, S. (2007). Inhibitors of the Proteasome Suppress Homologous DNA Recombination in Mammalian Cells. Cancer Res. 67: 8536-8543 [Abstract] [Full Text]  
• Jacquemont, C., Taniguchi, T. (2007). Proteasome Function Is Required for DNA Damage Response and Fanconi Anemia Pathway Activation. Cancer Res. 67: 7395-7405 [Abstract] [Full Text]  
• Bergink, S., Salomons, F. A., Hoogstraten, D., Groothuis, T. A.M., de Waard, H., Wu, J., Yuan, L., Citterio, E., Houtsmuller, A. B., Neefjes, J., Hoeijmakers, J. H.J., Vermeulen, W., Dantuma, N. P. (2006). DNA damage triggers nucleotide excision repair-dependent monoubiquitylation of histone H2A.. Genes Dev. 20: 1343-1352 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents