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Published online 1 May 2006. doi:10.1083/jcb.200510080
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 173, Number 3, 349-360
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Article

A functional network involved in the recycling of nucleocytoplasmic pre-60S factors



Alice Lebreton1, Cosmin Saveanu1, Laurence Decourty1, Jean-Christophe Rain2, Alain Jacquier1, and Micheline Fromont-Racine1

1 Génétique des Interactions Macromoléculaires, Centre National de la Recherche Scientifique URA2171, Institut Pasteur, 75724 Paris Cedex 15, France
2 Hybrigenics, 75014 Paris, France

Correspondence to Micheline Fromont-Racine: mfromont{at}pasteur.fr

Eukaryotic pre-ribosomes go through cytoplasmic maturation steps before entering translation. The nucleocytoplasmic proteins participating in these late stages of maturation are reimported to the nucleus. In this study, we describe a functional network focused on Rei1/Ybr267w, a strictly cytoplasmic pre-60S factor indirectly involved in nuclear 27S pre-ribosomal RNA processing. In the absence of Rei1, the nuclear import of at least three other pre-60S factors is impaired. The accumulation in the cytoplasm of a small complex formed by the association of Arx1 with a novel factor, Alb1/Yjl122w, inhibits the release of the putative antiassociation factor Tif6 from the premature large ribosomal subunits and its recycling to the nucleus. We propose a model in which Rei1 is a key factor for the coordinated dissociation and recycling of the last pre-60S factors before newly synthesized large ribosomal subunits enter translation.

Abbreviations used in this paper: PAP, peroxidase–antiperoxidase complex; rRNA, ribosomal RNA; TAP, tandem affinity purification; YPD, yeast extract–peptone–D-glucose.


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