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Published 8 May 2006. doi:10.1083/jcb.200506199
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 173, Number 3, 361-371
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Article

The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function



Alexis S. Madrid, Joel Mancuso, W. Zacheus Cande, and Karsten Weis

Division of Cell and Developmental Biology, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720

Correspondence to Karsten Weis: kweis{at}berkeley.edu

The nuclear pore complex (NPC) is a large channel that spans the two lipid bilayers of the nuclear envelope and mediates transport events between the cytoplasm and the nucleus. Only a few NPC components are transmembrane proteins, and the role of these proteins in NPC function and assembly remains poorly understood. We investigate the function of the three integral membrane nucleoporins, which are Ndc1p, Pom152p, and Pom34p, in NPC assembly and transport in Saccharomyces cerevisiae. We find that Ndc1p is important for the correct localization of nuclear transport cargoes and of components of the NPC. However, the role of Ndc1p in NPC assembly is partially redundant with Pom152p, as cells lacking both of these proteins show enhanced NPC disruption. Electron microscopy studies reveal that the absence of Ndc1p and Pom152p results in aberrant pores that have enlarged diameters and lack proteinaceous material, leading to an increased diffusion between the cytoplasm and the nucleus.

Abbreviations used in this paper: mRFP, monomeric red fluorescent protein; NES, nuclear export signal; NPC, nuclear pore complex; Nup, nucleoporin; POM, pore membrane protein; SPB, spindle pole body; TEM, transmission electron microscopy.


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