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Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells

¹ Zentrum für Molekulare Biologie der Universität Heidelberg, D-69120 Heidelberg, Germany
² Max-Planck-Institut für Biophysikalische Chemie, D-37707 Göttingen, Germany

Correspondence to Dirk Görlich: dg{at}zmbh.uni-heidelberg.de

So far, POM121 and gp210 are the only known anchoring sites of vertebrate nuclear pore complexes (NPCs) within the lipid bilayer of the nuclear envelope (NE) and, thus, are excellent candidates for initiating the NPC assembly process. Indeed, we demonstrate that POM121 can recruit several nucleoporins, such as Nup62 or Nup358, to ectopic assembly sites. It thus appears to act as a nucleation site for the assembly of NPC substructures. Nonetheless, we observed functional NPCs and intact NEs in severely POM121-depleted cells. Double knockdowns of gp210 and POM121 in HeLa cells, as well as depletion of POM121 from human fibroblasts, which do not express gp210, further suggest that NPCs can assemble or at least persist in a POM121- and gp210-free form. This points to extensive redundancies in protein–protein interactions within NPCs and suggests that vertebrate NPCs contain additional membrane-integral nucleoporins for anchorage within the lipid bilayer of the NE. In Stavru et al. (on p. 509 of this issue), we describe such an additional transmembrane nucleoporin as the metazoan orthologue of yeast Ndc1p.

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NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes

Fabrizia Stavru, Bastian B. Hülsmann, Anne Spang, Enno Hartmann, Volker C. Cordes, and Dirk Görlich
J. Cell Biol. 2006 173: 509-519. [Abstract] [Full Text] [PDF]

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