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Published online 12 June 2006. doi:10.1083/jcb.200602007
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 173, Number 6, 861-866
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 Plasma membrane polarization during mating in yeast cells

Tomasz J. Proszynski, Robin Klemm, Michel Bagnat, Katharina Gaus, and Kai Simons

Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany

Correspondence to Kai Simons: simons{at}mpi-cbg.de

The yeast mating cell provides a simple paradigm for analyzing mechanisms underlying the generation of surface polarity. Endocytic recycling and slow diffusion on the plasma membrane were shown to facilitate polarized surface distribution of Snc1p (Valdez-Taubas, J., and H.R. Pelham. 2003. Curr. Biol. 13:1636–1640). Here, we found that polarization of Fus1p, a raft-associated type I transmembrane protein involved in cell fusion, does not depend on endocytosis. Instead, Fus1p localization to the tip of the mating projection was determined by its cytosolic domain, which binds to peripheral proteins involved in mating tip polarization. Furthermore, we provide evidence that the lipid bilayer at the mating projection is more condensed than the plasma membrane enclosing the cell body, and that sphingolipids are required for this lipid organization.

M. Bagnat's present address is Department of Biochemistry and Biophysics, University of California, San Francisco. 513 Parnassus Avenue, San Francisco, CA 94143.

K. Gaus' present address is Centre for Vascular Research at the School of Medical Sciences, University of New South Wales, Sydney 2052, New South Wales, Australia.

Abbreviations used in this paper: GP, general polarization; SH, Src kinase homology; TMD, transmembrane domain.


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