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Published 19 June 2006. doi:10.1083/jcb.200511072
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 173, Number 6, 949-961
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Article

 The V0-ATPase mediates apical secretion of exosomes containing Hedgehog-related proteins in Caenorhabditis elegans

Samuel Liégeois, Alexandre Benedetto, Jean-Marie Garnier, Yannick Schwab, and Michel Labouesse

Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Universite Louis Pasteur, 67400 Illkirch, France

Correspondence to Michel Labouesse: lmichel{at}igbmc.u-strasbg.fr

Polarized intracellular trafficking in epithelia is critical in development, immunity, and physiology to deliver morphogens, defensins, or ion pumps to the appropriate membrane domain. The mechanisms that control apical trafficking remain poorly defined. Using Caenorhabditis elegans, we characterize a novel apical secretion pathway involving multivesicularbodies and the release of exosomes at the apical plasma membrane. By means of two different genetic approaches, we show that the membrane-bound V0 sector of the vacuolar H+-ATPase (V-ATPase) acts in this pathway, independent of its contribution to the V-ATPase proton pump activity. Specifically, we identified mutations in the V0 "a" subunit VHA-5 that affect either the V0-specific function or the V0+V1 function of the V-ATPase. These mutations allowed us to establish that the V0 sector mediates secretion of Hedgehog-related proteins. Our data raise the possibility that the V0 sector mediates exosome and morphogen release in mammals.

S. Liégeois and A. Benedetto contributed equally to this paper.

S. Liégeois's present address is Institut de Biologie Moléculaire et Cellulaire, F-67000 Strasbourg, France.

Abbreviations used in this paper: DIC, differential interference contrast; mRFP, monomeric red fluorescent protein; MVB, multivesicular body; SEM, scanning electron microscopy; TEM, transmission electron microscopy; V-ATPase, vacuolar H+-ATPase.


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