JCB logo
Carestream Gel Logic 212PRO
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 10 July 2006. doi:10.1083/jcb.200604011
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 174, Number 2, 221-229
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 779K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomas, S. G.
Right arrow Articles by Franklin-Tong, V. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomas, S. G.
Right arrow Articles by Franklin-Tong, V. E.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

Actin depolymerization is sufficient to induce programmed cell death in self-incompatible pollen



Steven G. Thomas1, Shanjin Huang2, Shutian Li1, Christopher J. Staiger2,3, and Vernonica E. Franklin-Tong1

1 School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, England, UK
2 Department of Biological Sciences and 3 Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907

Correspondence to Vernonica E. Franklin-Tong: v.e.franklin-tong{at}bham.ac.uk

Self-incompatibility (SI) prevents inbreeding through specific recognition and rejection of incompatible pollen. In incompatible Papaver rhoeas pollen, SI triggers a Ca2+ signaling cascade, resulting in the inhibition of tip growth, actin depolymerization, and programmed cell death (PCD). We investigated whether actin dynamics were implicated in regulating PCD. Using the actin-stabilizing and depolymerizing drugs jasplakinolide (Jasp) and latrunculin B, we demonstrate that changes in actin filament levels or dynamics play a functional role in initiating PCD in P. rhoeas pollen, triggering a caspase-3–like activity. Significantly, SI-induced PCD in incompatible pollen was alleviated by pretreatment with Jasp. This represents the first account of a specific causal link between actin polymerization status and initiation of PCD in a plant cell and significantly advances our understanding of the mechanisms involved in SI.

S.G. Thomas and S. Huang contributed equally to this paper.

Abbreviations used in this paper: CD, cytochalasin D; F-actin, filamentous actin; G-actin, globular actin; GM, germination medium; Jasp, jasplakinolide; LatB, latrunculin B; PCD, programmed cell death; ROS, reactive oxygen species; SI, self-incompatibility.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Actin senses self-incompatibility
Rabiya S. Tuma
J. Cell Biol. 2006 174: 165. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents