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Published 14 August 2006. doi:10.1083/jcb.200603069
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 174, Number 4, 491-497
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Perturbing integrin function inhibits microtubule growth from centrosomes, spindle assembly, and cytokinesis



Carlos G. Reverte, Angela Benware, Christopher W. Jones, and Susan E. LaFlamme

Center for Cell Biology and Cancer Research, Albany Medical College, Albany, NY 12208

Correspondence to Susan E. LaFlamme: laflams{at}mail.amc.edu

In many mammalian cell types, integrin-mediated cell-matrix adhesion is required for the G1–S transition of the cell cycle. As cells approach mitosis, a dramatic remodeling of their cytoskeleton accompanies dynamic changes in matrix adhesion, suggesting a mechanistic link. However, the role of integrins in cell division remains mostly unexplored. Using two cellular systems, we demonstrate that a point mutation in the ß1 cytoplasmic domain (ß1 tail) known to decrease integrin activity supports entry into mitosis but inhibits the assembly of a radial microtubule array focused at the centrosome during interphase, the formation of a bipolar spindle at mitosis and cytokinesis. These events are restored by externally activating the mutant integrin with specific antibodies. This is the first demonstration that the integrin ß1 tail can regulate centrosome function, the assembly of the mitotic spindle, and cytokinesis.

Abbreviations used in this paper: FA, focal adhesion; Fg, fibrinogen; Fn, fibronectin; Lm, laminin-1; MT, microtubule; WT, wild-type.


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