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Published online 21 August 2006. doi:10.1083/jcb.200601062
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 174, Number 5, 665-675
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Article

The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to connect the spindle pole body with the nuclear envelope



Sue L. Jaspersen1,2, Adriana E. Martin1, Galina Glazko1, Thomas H. Giddings, Jr.2, Garry Morgan2, Arcady Mushegian1, and Mark Winey2

1 Stowers Institute for Medical Research, Kansas City, MO 64110
2 Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309

Correspondence to Sue L. Jaspersen: slj{at}stowers-institute.org

The spindle pole body (SPB) is the sole site of microtubule nucleation in Saccharomyces cerevisiae; yet, details of its assembly are poorly understood. Integral membrane proteins including Mps2 anchor the soluble core SPB in the nuclear envelope. Adjacent to the core SPB is a membrane-associated SPB substructure known as the half-bridge, where SPB duplication and microtubule nucleation during G1 occurs. We found that the half-bridge component Mps3 is the budding yeast member of the SUN protein family (Sad1-UNC-84 homology) and provide evidence that it interacts with the Mps2 C terminus to tether the half-bridge to the core SPB. Mutants in the Mps3 SUN domain or Mps2 C terminus have SPB duplication and karyogamy defects that are consistent with the aberrant half-bridge structures we observe cytologically. The interaction between the Mps3 SUN domain and Mps2 C terminus is the first biochemical link known to connect the half-bridge with the core SPB. Association with Mps3 also defines a novel function for Mps2 during SPB duplication.

G. Glazko's present address is Department of Biostatistics and Computational Biology, University of Rochester Medical Center, Rochester, NY 14642.

G. Morgan's present address is Institute for Molecular Bioscience, University of Queensland, Brisbane QLD4072, Australia.

Abbreviations used in this paper: 5-FOA, 5-fluorootic acid; G6PDH, glucose-6-phosphate dehydrogenase; KASH, Klarsicht/ANC-1/Syne homology; MBP, maltose binding protein; SPB, spindle pole body; SUN, Sad1-Unc-84 homology; ts, temperature-sensitive.


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