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Published online 18 September 2006. doi:10.1083/jcb.200605004
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 174, Number 7, 997-1007
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Article

Ca2+ and synaptotagmin VII–dependent delivery of lysosomal membrane to nascent phagosomes



Cecilia Czibener1, Nathan M. Sherer1, Steven M. Becker1, Marc Pypaert2, Enfu Hui3,4, Edwin R. Chapman3,4, Walther Mothes1, and Norma W. Andrews1,2

1 Section of Microbial Pathogenesis and 2 Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510
3 Howard Hughes Medical Institute and 4 Department of Physiology, University of Wisconsin, Madison, WI 53706

Correspondence to Norma W. Andrews: norma.andrews{at}yale.edu

Synaptotagmin (Syt) VII is a ubiquitously expressed member of the Syt family of Ca2+ sensors. It is present on lysosomes in several cell types, where it regulates Ca2+-dependent exocytosis. Because [Ca2+]i and exocytosis have been associated with phagocytosis, we investigated the phagocytic ability of macrophages from Syt VII–/– mice. Syt VII–/– macrophages phagocytose normally at low particle/cell ratios but show a progressive inhibition in particle uptake under high load conditions. Complementation with Syt VII rescues this phenotype, but only when functional Ca2+-binding sites are retained. Reinforcing a role for Syt VII in Ca2+-dependent phagocytosis, particle uptake in Syt VII–/– macrophages is significantly less dependent on [Ca2+]i. Syt VII is concentrated on peripheral domains of lysosomal compartments, from where it is recruited to nascent phagosomes. Syt VII recruitment is rapidly followed by the delivery of Lamp1 to phagosomes, a process that is inhibited in Syt VII–/– macrophages. Thus, Syt VII regulates the Ca2+-dependent mobilization of lysosomes as a supplemental source of membrane during phagocytosis.

Abbreviations used in this paper: BMM, bone marrow macrophage; PC, phosphatidylcholine; PS, phosphatidylserine; Syt, synaptotagmin; VAMP, vesicle-associated membrane protein.


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