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Published 9 October 2006. doi:10.1083/jcb.200603039
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 175, Number 1, 77-85
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Article

MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206



Miriam I. Rosenberg1, Sara A. Georges1, Amy Asawachaicharn1, Erwin Analau1, and Stephen J. Tapscott1,2

1 Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109
2 Department of Neurology, University of Washington, Seattle, WA 98109

Correspondence to Stephen J. Tapscott: stapscot{at}fhcrc.org

Terminal differentiation of distinct cell types requires the transcriptional activation of differentiation-specific genes and the suppression of genes associated with the precursor cell. For example, the expression of utrophin (Utrn) is suppressed during skeletal muscle differentiation, and it is replaced at the sarcolemma by the related dystrophin protein. The MyoD transcription factor directly activates the expression of a large number of skeletal muscle genes, but also suppresses the expression of many genes. To characterize a mechanism of MyoD-mediated suppression of gene expression, we investigated two genes that are suppressed in fibroblasts converted to skeletal muscle by MyoD, follistatin-like 1 (Fstl1) and Utrn. MyoD directly activates the expression of a muscle-specific microRNA (miRNA), miR-206, which targets sequences in the Fstl1 and Utrn RNA, and these sequences are sufficient to suppress gene expression in the presence of miR-206. These findings demonstrate that MyoD, in addition to activating muscle-specific genes, induces miRNAs that repress gene expression during skeletal muscle differentiation.

M.I. Rosenberg and S.A. Georges contributed equally to this paper.

Abbreviations used in this paper: ChIP, chromatin immunoprecipitation; DM, differentiation medium; Fstl1, follistatin-like 1; MDER, MyoD estrogen receptor; MEF, mouse embryonic fibroblast; miRNA, microRNA.


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