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Published 6 November 2006. doi:10.1083/jcb.200604099
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 175, Number 3, 401-413
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Article

UV-induced fragmentation of Cajal bodies



Mario Cioce1, Séverine Boulon1, A. Gregory Matera2, and Angus I. Lamond1

1 Gene Regulation and Expression Division, University of Dundee, Dundee DD1 5EH, Scotland, UK
2 Department of Genetics, Case Western Reserve University School of Medicine, Cleveland, OH 44106

Correspondence to Angus I. Lamond: angus{at}lifesci.dundee.ac.uk

The morphology and composition of subnuclear organelles, such as Cajal bodies (CBs), nucleoli, and other nuclear bodies, is dynamic and can change in response to a variety of cell stimuli, including stress. We show that UV-C irradiation disrupts CBs and alters the distribution of a specific subset of CB components. The effect of UV-C on CBs differs from previously reported effects of transcription inhibitors. We demonstrate that the mechanism underlying the response of CBs to UV-C is mediated, at least in part, by PA28{gamma} (proteasome activator subunit {gamma}). The presence of PA28{gamma} in coilin-containing complexes is increased by UV-C. Overexpression of PA28{gamma}, in the absence of UV-C treatment, provokes a similar redistribution of the same subset of CB components that respond to UV-C. RNA interference–mediated knockdown of PA28{gamma} attenuates the nuclear disruption caused by UV-C. These data demonstrate that CBs are specific nuclear targets of cellular stress-response pathways and identify PA28{gamma} as a novel regulator of CB integrity.

Mario Cioce's present address is Instituto di Ricerca di Biologia Molecolare P. Angeletti, Merck Research Laboratories Rome, 00040 Pomezia Rome, Italy.

Abbreviations used in this paper: CB, Cajal body; FU, fluoruridine; IFN{gamma}, interferon {gamma}; DRB, 5,6-dichloro-1-ß-D-ribobenzimidazole; NB, nuclear body; RNAi, RNA interference; sDMA, symmetric dimethylarginine; SMN, survival of motor neuron; snRNP, small nuclear RNP; TMG, trimethylguanosine.


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