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Published online 30 October 2006. doi:10.1083/jcb.200607008
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 175, Number 3, 427-439
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Article

Spatial codes in dendritic BC1 RNA



Ilham A. Muslimov1, Anna Iacoangeli1, Jürgen Brosius3, and Henri Tiedge1,2

1 Department of Physiology and Pharmacology and 2 Department of Neurology, The Robert F. Furchgott Center for Neural and Behavioral Science, State University of New York Health Science Center at Brooklyn, Brooklyn, NY 11203
3 Institute of Experimental Pathology, Center for Molecular Biology of Inflammation, University of Münster, D-48149 Münster, Germany

Correspondence to Henri Tiedge: htiedge{at}downstate.edu

BC1 RNA is a dendritic untranslated RNA that has been implicated in local translational control mechanisms in neurons. Prerequisite for a functional role of the RNA in synaptodendritic domains is its targeted delivery along the dendritic extent. We report here that the targeting-competent 5' BC1 domain carries two dendritic targeting codes. One code, specifying somatic export, is located in the medial-basal region of the 5' BC1 stem-loop structure. It is defined by an export-determinant stem-bulge motif. The second code, specifying long-range dendritic delivery, is located in the apical part of the 5' stem-loop domain. This element features a GA kink-turn (KT) motif that is indispensable for distal targeting. It specifically interacts with heterogeneous nuclear ribonucleoprotein A2, a trans-acting targeting factor that has previously been implicated in the transport of MBP mRNA in oligodendrocytes and neurons. Our work suggests that a BC1 KT motif encodes distal targeting via the A2 pathway and that architectural RNA elements, such as KT motifs, may function as spatial codes in neural cells.

Abbreviations used in this paper: ANOVA, analysis of variance; CaMKII{alpha}, Ca2+/calmodulin-dependent kinase II {alpha} subunit; DTE, dendritic targeting element; EMSA, electrophoretic mobility shift assay; hnRNP, heterogeneous nuclear ribonucleoprotein; KT, kink-turn; MBP, myelin basic protein; PKM{zeta}, protein kinase M {zeta}; RRM, RNA recognition motif; rRNA, ribosomal RNA; WC, Watson–Crick.


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