JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 13 November 2006. doi:10.1083/jcb.200605092
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 175, Number 4, 547-554
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 2031K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schermer, B.
Right arrow Articles by Benzing, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schermer, B.
Right arrow Articles by Benzing, T.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Report

The von Hippel-Lindau tumor suppressor protein controls ciliogenesis by orienting microtubule growth



Bernhard Schermer1, Cristina Ghenoiu1, Malte Bartram1, Roman Ulrich Müller1, Fruzsina Kotsis1, Martin Höhne1, Wolfgang Kühn1, Manuela Rapka1, Roland Nitschke3, Hanswalter Zentgraf4, Manfred Fliegauf2, Heymut Omran2, Gerd Walz1, and Thomas Benzing1

1 Renal Division and 2 Children's Hospital, University Hospital Freiburg, 79106 Freiburg, Germany
3 Life Imaging Center, Institute of Biology, University of Freiburg, 79104 Freiburg, Germany
4 Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany

Correspondence to Thomas Benzing: thomas.benzing{at}uniklinik-freiburg.de

Cilia are specialized organelles that play an important role in several biological processes, including mechanosensation, photoperception, and osmosignaling. Mutations in proteins localized to cilia have been implicated in a growing number of human diseases. In this study, we demonstrate that the von Hippel-Lindau (VHL) protein (pVHL) is a ciliary protein that controls ciliogenesis in kidney cells. Knockdown of pVHL impeded the formation of cilia in mouse inner medullary collecting duct 3 kidney cells, whereas the expression of pVHL in VHL-negative renal cancer cells rescued the ciliogenesis defect. Using green fluorescent protein–tagged end-binding protein 1 to label microtubule plus ends, we found that pVHL does not affect the microtubule growth rate but is needed to orient the growth of microtubules toward the cell periphery, a prerequisite for the formation of cilia. Furthermore, pVHL interacts with the Par3–Par6–atypical PKC complex, suggesting a mechanism for linking polarity pathways to microtubule capture and ciliogenesis.

B. Schermer, C. Ghenoiu, and M. Bartram contributed equally to this paper.

Abbreviations used in this paper: aPKC, atypical PKC; EB1, end-binding protein 1; HEK, human embryonic kidney; IFT, intraflagellar transport; mIMCD, mouse inner medullary collecting duct; pVHL, VHL protein; RCC, renal cell carcinoma; shRNA, short hairpin RNA; UTR, untranslated region; VHL, von Hippel-Lindau.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Lining up microtubules
Mitch Leslie
J. Cell Biol. 2006 175: 517a. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents