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Published online 27 November 2006. doi:10.1083/jcb.200606017
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 175, Number 5, 791-802
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Article

Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane



Sabine Witzel1, Vitaly Zimyanin1, Filipa Carreira-Barbosa2, Masazumi Tada2, and Carl-Philipp Heisenberg1

1 Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
2 Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, England, UK

Correspondence to Carl-Philipp Heisenberg: Heisenberg{at}mpi-cbg.de; or Masazumi Tada: M.Tada{at}ucl.ac.uk

Wnt11 is a key signal, determining cell polarization and migration during vertebrate gastrulation. It is known that Wnt11 functionally interacts with several signaling components, the homologues of which control planar cell polarity in Drosophila melanogaster. Although in D. melanogaster these components are thought to polarize cells by asymmetrically localizing at the plasma membrane, it is not yet clear whether their subcellular localization plays a similarly important role in vertebrates. We show that in zebrafish embryonic cells, Wnt11 locally functions at the plasma membrane by accumulating its receptor, Frizzled 7, on adjacent sites of cell contacts. Wnt11-induced Frizzled 7 accumulations recruit the intracellular Wnt signaling mediator Dishevelled, as well as Wnt11 itself, and locally increase cell contact persistence. This increase in cell contact persistence is mediated by the local interaction of Wnt11, Frizzled 7, and the atypical cadherin Flamingo at the plasma membrane, and it does not require the activity of further downstream effectors of Wnt11 signaling, such as RhoA and Rok2. We propose that Wnt11, by interacting with Frizzled 7 and Flamingo, modulates local cell contact persistence to coordinate cell movements during gastrulation.

Abbreviations used in this paper: hpf, h after fertilization; MO, morpholino; PCP, planar cell polarity.


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