JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 18 December 2006. doi:10.1083/jcb.200608012
The Rockefeller University Press, 0021-9525 $8.00
JCB, Volume 175, Number 6, 971-980
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 5242K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chiba, H.
Right arrow Articles by Sawada, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chiba, H.
Right arrow Articles by Sawada, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

The nuclear receptor hepatocyte nuclear factor 4{alpha} acts as a morphogen to induce the formation of microvilli



Hideki Chiba1, Naoyuki Sakai1, Masaki Murata1, Makoto Osanai1, Takafumi Ninomiya2, Takashi Kojima1, and Norimasa Sawada1

1 Department of Pathology and 2 Department of Anatomy, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan

Correspondence to Hideki Chiba: hidchiba{at}sapmed.ac.jp

Microvilli are actin-based organelles found on apical plasma membranes that are involved in nutrient uptake and signal transduction. Numerous components, including ezrin/radixin/moesin (ERM) proteins, have been identified that link filamentous actins to transmembrane proteins, but the signals driving microvillus biogenesis are not known. In this study, we show that the conditional and/or ectopic expression of a nuclear receptor, hepatocyte nuclear factor 4{alpha} (HNF4{alpha}), triggers microvillus morphogenesis. We also demonstrate that HNF4{alpha} expression induces ERM-binding phosphoprotein 50 (EBP50) expression and that attenuation of EBP50 using RNA interference inhibits microvillus development. We conclude that HNF4{alpha} acts as a morphogen to trigger microvillus formation.

Abbreviations used in this paper: Dox, doxycycline; EBP50, ERM-binding phosphoprotein 50; ERM, ezrin/radixin/moesin; HNF4{alpha}, hepatocyte nuclear factor 4{alpha}; RAR{gamma}, retinoic acid receptor {gamma}; RLE, rat lung endothelial; RXR{alpha}, retinoid X receptor {alpha}; tRA, all-trans retinoic acid; WT, wild type.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents