JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
A correction to this article has been published: Tamada et al., J. Cell Biol. 176 (4) 545
Published online January 2, 2007
doi:10.1083/jcb.200609116
The Journal of Cell Biology, Vol. 176, No. 1, 27-33
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Tamada et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 5824K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Correction (v176,p545)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tamada, M.
Right arrow Articles by Sheetz, M. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tamada, M.
Right arrow Articles by Sheetz, M. P.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Report

Two distinct modes of myosin assembly and dynamics during epithelial wound closure



Masako Tamada1, Tomas D. Perez2, W. James Nelson2, and Michael P. Sheetz1

1 Department of Biological Sciences, Columbia University, New York, NY 10027
2 Department of Biological Sciences and Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305

Correspondence to Michael P. Sheetz: ms2001{at}columbia.edu

Actomyosin contraction powers the sealing of epithelial sheets during embryogenesis and wound closure; however, the mechanisms are poorly understood. After laser ablation wounding of Madin–Darby canine kidney cell monolayers, we observed distinct steps in wound closure from time-lapse images of myosin distribution during resealing. Immediately upon wounding, actin and myosin II regulatory light chain accumulated at two locations: (1) in a ring adjacent to the tight junction that circumscribed the wound and (2) in fibers at the base of the cell in membranes extending over the wound site. Rho-kinase activity was required for assembly of the myosin ring, and myosin II activity was required for contraction but not for basal membrane extension. As it contracted, the myosin ring moved toward the basal membrane with ZO-1 and Rho-kinase. Thus, we suggest that tight junctions serve as attachment points for the actomyosin ring during wound closure and that Rho-kinase is required for localization and activation of the contractile ring.

M. Tamada's present address is Kobe University Graduate School of Medicine, Chuo-ku, Kobe 650-0017 Hyogo, Japan.

T.D. Perez's present address is Department of Biological Sciences, Columbia University, New York, NY 10027.

Abbreviation used in this paper: MLC, myosin II regulatory light chain.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

A dual need for myosin in wounds
Nicole LeBrasseur
J. Cell Biol. 2007 176: 3. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents