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Published online
doi:10.1083/jcb.200604141
The Journal of Cell Biology, Vol. 176, No. 3, 283-294
The Rockefeller University Press, 0021-9525 $30.00
© Govin et al.
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Article

Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis



Jérôme Govin1,2, Emmanuelle Escoffier1,2, Sophie Rousseaux1,2, Lauriane Kuhn2,3,4, Myriam Ferro2,3,4, Julien Thévenon1,2, Raffaella Catena5, Irwin Davidson5, Jérôme Garin3,4, Saadi Khochbin1,2, and Cécile Caron1,2

1 Institut National de la Santé et de la Recherche Médicale, U309, Institut Albert Bonniot, F-38700 Grenoble, France
2 Université Joseph Fourier, F-38700 Grenoble, France
3 Commissariat à l'Energie Atomique/Grenoble, F-38054 Grenoble, France
4 Institut National de la Santé et de la Recherche Médicale, ERM0201, F-38054 Grenoble, France
5 Institut de Génétique et Biologie Moléculaire et Cellulaire/Centre National de la Recherche Scientifique/Institut National de la Santé et de la Recherche Médicale/Université Louis Pasteur, F-67404 Illkirch, France

Correspondence to Cécile Caron: Cecile.Caron{at}ujf-grenoble.fr; or Saadi Khochbin: Khochbin{at}ujf-grenoble.fr

During male germ cell postmeiotic maturation, dramatic chromatin reorganization occurs, which is driven by completely unknown mechanisms. For the first time, we describe a specific reprogramming of mouse pericentric heterochromatin. Initiated when histones undergo global acetylation in early elongating spermatids, this process leads to the establishment of new DNA packaging structures organizing the pericentric regions in condensing spermatids. Five new histone variants were discovered, which are expressed in late spermiogenic cells. Two of them, which we named H2AL1 and H2AL2, specifically mark the pericentric regions in condensing spermatids and participate in the formation of new nucleoprotein structures. Moreover, our investigations also suggest that TH2B, an already identified testis-specific H2B variant of unknown function, could provide a platform for the structural transitions accompanying the incorporation of these new histone variants.

J. Govin and E. Escoffier contributed equally to this paper.

Abbreviations used in this paper: IF, immunofluorescence; IH, immunohistochemistry; MS, mass spectrometry; qPCR, quantitative PCR.


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