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Published online February 20, 2007
doi:10.1083/jcb.200608041
The Journal of Cell Biology, Vol. 176, No. 5, 653-665
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Hou et al.
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Article

 Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella

Yuqing Hou1, Hongmin Qin3, John A. Follit2, Gregory J. Pazour2, Joel L. Rosenbaum3, and George B. Witman1

1 Department of Cell Biology and 2 Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01655
3 Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520

Correspondence to George Witman: george.witman{at}umassmed.edu

Intraflagellar transport (IFT), which is the bidirectional movement of particles within flagella, is required for flagellar assembly. IFT particles are composed of ~16 proteins, which are organized into complexes A and B. We have cloned Chlamydomonas reinhardtii and mouse IFT46, and show that IFT46 is a highly conserved complex B protein in both organisms. A C. reinhardtii insertional mutant null for IFT46 has short, paralyzed flagella lacking dynein arms and with central pair defects. The mutant has greatly reduced levels of most complex B proteins, indicating that IFT46 is necessary for complex B stability. A partial suppressor mutation restores flagellar length to the ift46 mutant. IFT46 is still absent, but levels of the other IFT particle proteins are largely restored, indicating that complex B is stabilized in the suppressed strain. Axonemal ultrastructure is restored, except that the outer arms are still missing, although outer arm subunits are present in the cytoplasm. Thus, IFT46 is specifically required for transporting outer arms into the flagellum.

Abbreviation used in this paper: IFT, intraflagellar transport.


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