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Published online
doi:10.1083/jcb.200606134
The Journal of Cell Biology, Vol. 176, No. 6, 771-777
The Rockefeller University Press, 0021-9525 $30.00
© He et al.
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Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth



Bing He1, Fengong Xi1, Jian Zhang1, Daniel TerBush2, Xiaoyu Zhang1, and Wei Guo1

1 Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
2 Department of Biochemistry, Uniformed Services University of Health Sciences, Bethesda, MD 20814

Correspondence to Wei Guo: guowei{at}sas.upenn.edu

In budding yeast, two classes of post-Golgi secretory vesicles carrying different sets of cargoes typified by Bgl2p and invertase are delivered to the plasma membrane for secretion. The exocyst is implicated in tethering these vesicles to the daughter cell membrane for exocytosis. In this study, we report that mutations in the exocyst component Exo70p predominantly block secretion of the Bgl2p vesicles. Furthermore, a defect in invertase vesicle trafficking caused by vps1{Delta} or pep12{Delta} in the exo70 mutant background is detrimental to the cell. The secretion defect in exo70 mutants was most pronounced during the early budding stage, which affected daughter cell growth. The selective secretion block does not occur at the vesicle formation or sorting stage because the exocytic vesicles are properly generated and protein processing is normal in the exo70 mutants. Our study suggests that Exo70p functions primarily at early stages of the cell cycle in Bgl2p vesicle secretion, which is critical for polarized cell growth.

B. He and F. Xi contributed equally to this paper.

Abbreviations used in this paper: 5-FOA, 5-fluoroorotic acid; CPY, carboxypeptidase Y; SC, synthetic complete media; YPD, yeast extract/peptone/glucose.


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