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Published online
doi:10.1083/jcb.200611147
The Journal of Cell Biology, Vol. 176, No. 7, 1007-1019
The Rockefeller University Press, 0021-9525 $30.00
© Vascotto et al.
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Article

The actin-based motor protein myosin II regulates MHC class II trafficking and BCR-driven antigen presentation



Fulvia Vascotto1, Danielle Lankar1, Gabrielle Faure-André1, Pablo Vargas1, Jheimmy Diaz1, Delphine Le Roux1, Maria-Isabel Yuseff1, Jean-Baptiste Sibarita2, Marianne Boes3, Graça Raposo2, Evelyne Mougneau4, Nicolas Glaichenhaus4, Christian Bonnerot1, Bénédicte Manoury1, and Ana-Maria Lennon-Duménil1

1 Institut National de la Santé et de la Recherche Medicale Unité 653 and 2 Centre National de la Recherche Scientifique, Unité Mixte de Recherche 144 Institut Curie, 75005 Paris, France
3 Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115
4 Institut National de la Santé et de la Recherche Medicale E0344, Université de Nice-Sophia Antipolis, Institut de Pharmacologie Moléculaire et Cellulaire, 06560 Valbonne, France

Correspondence to Ana-Maria Lennon-Duménil: ana-maria.lennon{at}curie.fr

Antigen (Ag) capture and presentation onto major histocompatibility complex (MHC) class II molecules by B lymphocytes is mediated by their surface Ag receptor (B cell receptor [BCR]). Therefore, the transport of vesicles that carry MHC class II and BCR–Ag complexes must be coordinated for them to converge for processing. In this study, we identify the actin-associated motor protein myosin II as being essential for this process. Myosin II is activated upon BCR engagement and associates with MHC class II–invariant chain complexes. Myosin II inhibition or depletion compromises the convergence and concentration of MHC class II and BCR–Ag complexes into lysosomes devoted to Ag processing. Accordingly, the formation of MHC class II–peptides and subsequent CD4 T cell activation are impaired in cells lacking myosin II activity. Therefore, myosin II emerges as a key motor protein in BCR-driven Ag processing and presentation.

Dr. Bonnerot died on 26 May 2004.

Abbreviations used in this study: Ab, antibody; Ag, antigen; BCR, B cell receptor; DC, dendritic cell; LPS, lipopolysaccharide; MHC, major histocompatibility complex; MLC, myosin II light chain; NP, nanoparticle; OVA, ovalbumin.


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