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Published online
doi:10.1083/jcb.200607019
The Journal of Cell Biology, Vol. 176, No. 7, 1049-1060
The Rockefeller University Press, 0021-9525 $30.00
© Kuribayashi et al.
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Article

Essential role of protein kinase C {zeta} in transducing a motility signal induced by superoxide and a chemotactic peptide, fMLP



Kageaki Kuribayashi1, Kiminori Nakamura1,2, Maki Tanaka1, Tsutomu Sato1, Junji Kato1, Katsunori Sasaki3, Rishu Takimoto1, Katsuhisa Kogawa1, Takeshi Terui1, Tetsuji Takayama1, Takayuki Onuma1, Takuya Matsunaga1, and Yoshiro Niitsu1

1 Fourth Department of Internal Medicine, 2 Department of Molecular Medicine, Sapporo Medical University School of Medicine, Chuo-ku, Sapporo 060-8543, Japan
3 Core Facility for Therapeutic Vectors, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan

Correspondence to Yoshiro Niitsu: niitsu{at}sapmed.ac.jp

Under various pathological conditions, including infection, malignancy, and autoimmune diseases, tissues are incessantly exposed to reactive oxygen species produced by infiltrating inflammatory cells. We show augmentation of motility associated with morphological changes of human squamous carcinoma SASH1 cells, human peripheral monocytes (hPMs), and murine macrophage-like cell line J774.1 by superoxide stimulation. We also disclose that motility of hPMs and J774.1 induced by a chemotactic peptide (N-formyl-methionyl-leucyl-phenylalanine [fMLP]) was inhibited by superoxide dismutase or N-acetylcystein, indicating stimulation of motility by superoxide generated by fMLP stimulation. In these cells, protein kinase C (PKC) {zeta} was activated to phosphorylate RhoGDI-1, which liberated RhoGTPases, leading to their activation. These events were inhibited by dominant-negative PKC{zeta} in SASH1 cells, myristoylated PKC{zeta} peptides in hPMs and J774.1, or a specific inhibitor of RhoGTPase in SASH1, hPMs, and J774.1. These results suggest a new approach for manipulation of inflammation as well as tumor cell invasion by targeting this novel signaling pathway.

K. Kuribayashi and K. Nakamura contributed equally to this paper.

Abbreviations used in this paper: DHR, dihydrorhodamine; DN, dominant-negative; DPI, diphenylene iodonium; fMLP, N-formyl-methionyl-leucyl-phenylalanine; hPM, human peripheral monocyte; HPX, hypoxanthine; IP, immunoprecipitation; NAC, N-acetylcystein; ROS, reactive oxygen species; SOD, superoxide dismutase; XOD, xanthine oxidase.


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