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Published online
doi:10.1083/jcb.200612139
The Journal of Cell Biology, Vol. 177, No. 3, 551-562
The Rockefeller University Press, 0021-9525 $30.00
© Eshed et al.
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Article

Secreted gliomedin is a perinodal matrix component of peripheral nerves



Yael Eshed1, Konstantin Feinberg1, David J. Carey2, and Elior Peles1

1 Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
2 Weis Center for Research, Geisinger Clinic, Danville, PA 17822

Correspondence to Elior Peles: peles{at}weizmann.ac.il

The interaction between gliomedin and the axonodal cell adhesion molecules (CAMs) neurofascin and NrCAM induces the clustering of Na+ channels at the nodes of Ranvier. We define new interactions of gliomedin that are essential for its clustering activity. We show that gliomedin exists as both transmembrane and secreted forms that are generated by proteolytic cleavage of the protein, and that only the latter is detected at the nodes of Ranvier. The secreted extracellular domain of gliomedin binds to Schwann cells and is incorporated into the extracellular matrix (ECM) in a heparin-dependent manner, suggesting the involvement of heparan sulfate proteoglycans (HSPGs). Furthermore, we show that the N-terminal region of gliomedin serves as an oligomerization domain that mediates self-association of the molecule, which is required for its binding to neurofascin and NrCAM. Our results indicate that the deposition of gliomedin multimers at the nodal gap by binding to HSPGs facilitates the clustering of the axonodal CAMs and Na+ channels.

Abbreviations used in this paper: CAM, cell adhesion molecule; DRG, dorsal root ganglion; HEK, human embryonic kidney; HSPG, heparan sulfate proteoglycan; PNS, peripheral nervous system.


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