JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online
doi:10.1083/jcb.200701006
The Journal of Cell Biology, Vol. 177, No. 4, 683-694
The Rockefeller University Press, 0021-9525 $30.00
© Grande-García et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 2013K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grande-García, A.
Right arrow Articles by del Pozo, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grande-García, A.
Right arrow Articles by del Pozo, M. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Article

Caveolin-1 regulates cell polarization and directional migration through Src kinase and Rho GTPases



Araceli Grande-García1, Asier Echarri1, Johan de Rooij2, Nazilla B. Alderson1, Clare M. Waterman-Storer2, José M. Valdivielso1, and Miguel A. del Pozo1

1 Integrin Signaling Laboratory, Department of Vascular Biology and Inflammation, Centro Nacional de Investigaciones Cardiovasculares, 28029 Madrid, Spain
2 Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037

Correspondence to Miguel A. del Pozo: madelpozo{at}cnic.es

Development, angiogenesis, wound healing, and metastasis all involve the movement of cells in response to changes in the extracellular environment. To determine whether caveolin-1 plays a role in cell migration, we have used fibroblasts from knockout mice. Caveolin-1–deficient cells lose normal cell polarity, exhibit impaired wound healing, and have decreased Rho and increased Rac and Cdc42 GTPase activities. Directional persistency of migration is lost, and the cells show an impaired response to external directional stimuli. Both Src inactivation and p190RhoGAP knockdown restore the wild-type phenotype to caveolin-1–deficient cells, suggesting that caveolin-1 stimulates normal Rho GTP loading through inactivation of the Src–p190RhoGAP pathway. These findings highlight the importance of caveolin-1 in the establishment of cell polarity during directional migration through coordination of the signaling of Src kinase and Rho GTPases.

J. de Rooij's present address is Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands.

J.M. Valdivielso's present address is Hospital Universitario Arnau de Vilanova, Universidad de Lleida, 25198 Lleida, Spain.

Abbreviations used in this paper: Csk, C-terminal Src kinase; EF, elliptical factor; FA, focal adhesion; Fn, fibronectin; ID, index of directionality; MEF, mouse embryonic fibroblast; MTOC, microtubule organizing center; shRNA, short hairpin RNA; WT, wild-type.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents