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Published online
doi:10.1083/jcb.200701111
The Journal of Cell Biology, Vol. 177, No. 5, 843-855
The Rockefeller University Press, 0021-9525 $30.00
© Besse et al.
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Article

The Ig cell adhesion molecule Basigin controls compartmentalization and vesicle release at Drosophila melanogaster synapses



Florence Besse1, Sara Mertel2,3, Robert J. Kittel2,3, Carolin Wichmann2,3, Tobias M. Rasse2,4, Stephan J. Sigrist2,3, and Anne Ephrussi1

1 Developmental Biology Unit, European Molecular Biology Laboratory, D-69117 Heidelberg, Germany
2 European Neuroscience Institute Göttingen, D-37077 Göttingen, Germany
3 Institut für Klinische Neurobiologie und Rudolf-Virchow-Zentrum, Universität Würzburg, D-97078 Würzburg, Germany
4 Hertie-Institute for Clinical Brain Research, University of Tübingen, D-72076 Tübingen, Germany

Correspondence to Stephan J. Sigrist: stephan.sigrist{at}virchow.uni-wuerzburg.de; or Anne Ephrussi: ephrussi{at}embl.de

Synapses can undergo rapid changes in size as well as in their vesicle release function during both plasticity processes and development. This fundamental property of neuronal cells requires the coordinated rearrangement of synaptic membranes and their associated cytoskeleton, yet remarkably little is known of how this coupling is achieved. In a GFP exon-trap screen, we identified Drosophila melanogaster Basigin (Bsg) as an immunoglobulin domain-containing transmembrane protein accumulating at periactive zones of neuromuscular junctions. Bsg is required pre- and postsynaptically to restrict synaptic bouton size, its juxtamembrane cytoplasmic residues being important for that function. Bsg controls different aspects of synaptic structure, including distribution of synaptic vesicles and organization of the presynaptic cortical actin cytoskeleton. Strikingly, bsg function is also required specifically within the presynaptic terminal to inhibit nonsynchronized evoked vesicle release. We thus propose that Bsg is part of a transsynaptic complex regulating synaptic compartmentalization and strength, and coordinating plasma membrane and cortical organization.

Abbreviations used in this paper: BRP, Bruchpilot; CAM, cell adhesion molecule; CSP, cysteine string protein; eEJC, nerve-evoked excitatory junctional current; NMJ, neuromuscular junction; PSD, postsynaptic density; SSR, subsynaptic reticulum.


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