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Published online
doi:10.1083/jcb.200702145
The Journal of Cell Biology, Vol. 177, No. 6, 981-993
The Rockefeller University Press, 0021-9525 $30.00
© Khmelinskii et al.
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Article

Cdc14-regulated midzone assembly controls anaphase B



Anton Khmelinskii1, Clare Lawrence2, Johanna Roostalu1, and Elmar Schiebel1

1 Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany
2 The Paterson Institute for Cancer Research, Manchester M20 4BX, England, UK

Correspondence to Elmar Schiebel: e.schiebel{at}zmbh.uni-heidelberg.de

Spindle elongation in anaphase of mitosis is a cell cycle–regulated process that requires coordination between polymerization, cross-linking, and sliding of microtubules (MTs). Proteins that assemble at the spindle midzone may be important for this process. In this study, we show that Ase1 and the separase–Slk19 complex drive midzone assembly in yeast. Whereas the conserved MT-bundling protein Ase1 establishes a midzone, separase–Slk19 is required to focus and center midzone components. An important step leading to spindle midzone assembly is the dephosphorylation of Ase1 by the protein phosphatase Cdc14 at the beginning of anaphase. Failure to dephosphorylate Ase1 delocalizes midzone proteins and delays the second, slower phase of anaphase B. In contrast, in cells expressing nonphosphorylated Ase1, anaphase spindle extension is faster, and spindles frequently break. Cdc14 also controls the separase–Slk19 complex indirectly via the Aurora B kinase. Thus, Cdc14 regulates spindle midzone assembly and function directly through Ase1 and indirectly via the separase–Slk19 complex.

A. Khmelinskii and C. Lawrence contributed equally to this paper.

Abbreviations used in this paper: APC, anaphase-promoting complex; Cdk, cyclin-dependent kinase; MT, microtubule; SPB, spindle pole body; tdTomato, tandem-dimer Tomato; WT, wild type.


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