JCB logo
MBoC5 from Garland Science
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online July 16, 2007
doi:10.1083/jcb.200701078
The Journal of Cell Biology, Vol. 178, No. 2, 297-308
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Sun et al.
This Article
Right arrow Full Text
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sun, Y.
Right arrow Articles by Anderson, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sun, Y.
Right arrow Articles by Anderson, R. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

 Type I{gamma} phosphatidylinositol phosphate kinase is required for EGF-stimulated directional cell migration

Yue Sun1, Kun Ling1, Matthew P. Wagoner2, and Richard A. Anderson2

1 Department of Pharmacology, School of Medicine and Public Health, and 2 Program in Molecular and Cellular Pharmacology, University of Wisconsin–Madison, Madison, WI 53706

Correspondence to Richard A. Anderson: raanders{at}wisc.edu

Phosphatidylinositol 4,5-bisphosphate (PI4,5P2) modulates a plethora of cytoskeletal interactions that control the dynamics of actin assembly and, ultimately, cell migration. We show that the type I{gamma} phosphatidylinositol phosphate kinase 661 (PIPKI{gamma}661), an enzyme that generates PI4,5P2, is required for growth factor but not G protein–coupled receptor–stimulated directional migration. By generating PI4,5P2 and regulating talin assembly, PIPKI{gamma}661 modulates nascent adhesion formation at the leading edge to facilitate cell migration. The epidermal growth factor (EGF) receptor directly phosphorylates PIPKI{gamma}661 at tyrosine 634, and this event is required for EGF-induced migration. This phosphorylation regulates the interaction between PIPKI{gamma}661 and phospholipase C{gamma}1 (PLC{gamma}1, an enzyme previously shown to be involved in the regulation of EGF-stimulated migration). Our results suggest that phosphorylation events regulating specific PIPKI{gamma}661 interactions are required for growth factor–induced migration. These interactions in turn define the spatial and temporal generation of PI4,5P2 and derived messengers required for directional migration.

Abbreviations used in this paper: AP, adaptor protein; EGFR, EGF receptor; HGF, hepatocyte growth factor; LPA, lysophosphatidic acid; PI4,5P2, phosphatidylinositol 4,5-bisphosphate; PIPKI, type I phosphatidylinositol phosphate kinase; SDF, stromal cell–derived factor; shRNA, short hairpin RNA.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents