|
||
Article |
phosphatidylinositol phosphate kinase is required for EGF-stimulated directional cell migration
Correspondence to Richard A. Anderson: raanders{at}wisc.edu
Phosphatidylinositol 4,5-bisphosphate (PI4,5P2) modulates a plethora of cytoskeletal interactions that control the dynamics of actin assembly and, ultimately, cell migration. We show that the type I
phosphatidylinositol phosphate kinase 661 (PIPKI
661), an enzyme that generates PI4,5P2, is required for growth factor but not G proteincoupled receptorstimulated directional migration. By generating PI4,5P2 and regulating talin assembly, PIPKI
661 modulates nascent adhesion formation at the leading edge to facilitate cell migration. The epidermal growth factor (EGF) receptor directly phosphorylates PIPKI
661 at tyrosine 634, and this event is required for EGF-induced migration. This phosphorylation regulates the interaction between PIPKI
661 and phospholipase C
1 (PLC
1, an enzyme previously shown to be involved in the regulation of EGF-stimulated migration). Our results suggest that phosphorylation events regulating specific PIPKI
661 interactions are required for growth factorinduced migration. These interactions in turn define the spatial and temporal generation of PI4,5P2 and derived messengers required for directional migration. ![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati What's this?
This article has been cited by other articles:
|
|