JCB logo
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online
doi:10.1083/jcb.200609096
The Journal of Cell Biology, Vol. 178, No. 3, 387-398
The Rockefeller University Press, 0021-9525 $30.00
© Fan et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 6034K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fan, S.
Right arrow Articles by Margolis, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fan, S.
Right arrow Articles by Margolis, B.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*Protein
*UniGene
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Article

A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin ß interactions



Shuling Fan1, Vanessa Fogg1, Qian Wang2, Xiao-Wei Chen3, Chia-Jen Liu1, and Ben Margolis1,2

1 Department of Internal Medicine, 2 Department of Biological Chemistry, and 3 Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109

Correspondence to Ben Margolis: bmargoli{at}umich.edu

The Crumbs family of apical transmembrane proteins regulates apicobasal polarity via protein interactions with a conserved C-terminal sequence, ERLI. However, one of the mammalian Crumbs proteins, Crumbs3 (CRB3) has an alternate splice form with a novel C-terminal sequence ending in CLPI (CRB3-CLPI). We report that CRB3-CLPI localizes to the cilia membrane and a membrane compartment at the mitotic spindle poles. Knockdown of CRB3-CLPI leads to both a loss of cilia and a multinuclear phenotype associated with centrosomal and spindle abnormalities. Using protein purification, we find that CRB3-CLPI interacts with importin ß-1 in a Ran-regulated fashion. Importin ß-1 colocalizes with CRB3-CLPI during mitosis, and a dominant-negative form of importin ß-1 closely phenocopies CRB3-CLPI knockdown. Knockdown of importin ß-1 blocks targeting of CRB3-CLPI to the spindle poles. Our data suggest an expanded role for Crumbs proteins in polarized membrane targeting and cell division via unique interactions with importin proteins.

Abbreviations used in this paper: EST, expressed sequence tag; shRNA, short hairpin RNA; wt, wild-type.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents