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Published online July 23, 2007
doi:10.1083/jcb.200702167
The Journal of Cell Biology, Vol. 178, No. 3, 399-410
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Motley et al.
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Yeast peroxisomes multiply by growth and division



Alison M. Motley and Ewald H. Hettema

Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, England, UK

Correspondence to Ewald H. Hettema: e.hettema{at}sheffield.ac.uk

Peroxisomes can arise de novo from the endoplasmic reticulum (ER) via a maturation process. Peroxisomes can also multiply by fission. We have investigated how these modes of multiplication contribute to peroxisome numbers in Saccharomyces cerevisiae and the role of the dynamin-related proteins (Drps) in these processes. We have developed pulse-chase and mating assays to follow the fate of existing peroxisomes, de novo–formed peroxisomes, and ER-derived preperoxisomal structures. We find that in wild-type (WT) cells, peroxisomes multiply by fission and do not form de novo. A marker for the maturation pathway, Pex3-GFP, is delivered from the ER to existing peroxisomes. Strikingly, cells lacking peroxisomes as a result of a segregation defect do form peroxisomes de novo. This process is slower than peroxisome multiplication in WT cells and is Drp independent. In contrast, peroxisome fission is Drp dependent. Our results show that peroxisomes multiply by growth and division under our assay conditions. We conclude that the ER to peroxisome pathway functions to supply existing peroxisomes with essential membrane constituents.

Abbreviations used in this paper: Drp, dynamin-related protein; PTS1, peroxisome-targeting signal type 1; WT, wild type.


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