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Published online
doi:10.1083/jcb.200704081
The Journal of Cell Biology, Vol. 178, No. 5, 725-732
The Rockefeller University Press, 0021-9525 $30.00
© Lucas et al.
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Maintaining the proper connection between the centrioles and the pericentriolar matrix requires Drosophila Centrosomin



Eliana P. Lucas and Jordan W. Raff

Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, England, UK

Correspondence to Jordan W. Raff: j.raff{at}gurdon.cam.ac.uk

Centrosomes consist of two centrioles surrounded by an amorphous pericentriolar matrix (PCM), but it is unknown how centrioles and PCM are connected. We show that the centrioles in Drosophila embryos that lack the centrosomal protein Centrosomin (Cnn) can recruit PCM components but cannot maintain a proper attachment to the PCM. As a result, the centrioles "rocket" around in the embryo and often lose their connection to the nucleus in interphase and to the spindle poles in mitosis. This leads to severe mitotic defects in embryos and to errors in centriole segregation in somatic cells. The Cnn-related protein CDK5RAP2 is linked to microcephaly in humans, but cnn mutant brains are of normal size, and we observe only subtle defects in the asymmetric divisions of mutant neuroblasts. We conclude that Cnn maintains the proper connection between the centrioles and the PCM; this connection is required for accurate centriole segregation in somatic cells but is not essential for the asymmetric division of neuroblasts.

Abbreviations used in this paper: Cnn, centrosomin; GSC, germline stem cell; MT, microtubule; MTOC, MT organizing center; NB, neuroblast; NEB, nuclear envelope breakdown; PCM, pericentriolar matrix; WT, wild-type.


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