JCB logo
ReproCELL
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
A correction to this article has been published: Zhang et al., J. Cell Biol. 179 (2) 357
Published online August 20, 2007
doi:10.1083/jcb.200702138
The Journal of Cell Biology, Vol. 178, No. 5, 773-784
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Zhang et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 6474K)
Right arrow PPT slides of all figures
Right arrow Correction (v179,p357)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, J.
Right arrow Articles by Mao, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, J.
Right arrow Articles by Mao, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment



Jiayin Zhang, Sana Ahmad, and Yinghui Mao

Department of Pathology and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032

Correspondence to Yinghui Mao: ym2183{at}columbia.edu

The accurate segregation of chromosomes in mitosis requires the stable attachment of microtubules to kinetochores. The details of this complex and dynamic process are poorly understood. In this study, we report the interaction of a kinetochore-associated mitotic checkpoint kinase, BubR1, with two microtubule plus end–associated proteins, adenomatous polyposis coli (APC) and EB1, providing a potential link in stable kinetochore microtubule attachment. Using immunodepletion from and antibody addition to Xenopus laevis egg extracts, we show that BubR1 and its kinase activity are essential for positioning chromosomes at the metaphase plate. BubR1 associates with APC and EB1 in egg extracts, and the complex formation is necessary for metaphase chromosome alignment. Using purified components, BubR1 directly phosphorylates APC and forms a ternary complex with APC and microtubules. These findings support a model in which BubR1 kinase may directly regulate APC function involved in stable kinetochore microtubule attachment.

Abbreviations used in this paper: APC, adenomatous polyposis coli; CENP, centromere protein; CSF, cytostatic factor; KD, kinase dead; MCAK, mitotic centromere-associated kinesin; NTA, nitrilotriacetic acid; WT, wild type.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents