JCB logo
R&D Systems: New Poster Available
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online August 27, 2007
doi:10.1083/jcb.200702120
The Journal of Cell Biology, Vol. 178, No. 5, 799-812
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Scarcelli et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 2660K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Scarcelli, J. J.
Right arrow Articles by Cole, C. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scarcelli, J. J.
Right arrow Articles by Cole, C. N.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

The yeast integral membrane protein Apq12 potentially links membrane dynamics to assembly of nuclear pore complexes



John J. Scarcelli1, Christine A. Hodge1, and Charles N. Cole1,2

1 Department of Biochemistry and 2 Department of Genetics, Dartmouth Medical School, Hanover, NH 03755

Correspondence to Charles N. Cole: charles.cole{at}dartmouth.edu

Although the structure and function of components of the nuclear pore complex (NPC) have been the focus of many studies, relatively little is known about NPC biogenesis. In this study, we report that Apq12 is required for efficient NPC biogenesis in Saccharomyces cerevisiae. Apq12 is an integral membrane protein of the nuclear envelope (NE) and endoplasmic reticulum. Cells lacking Apq12 are cold sensitive for growth, and a subset of their nucleoporins (Nups), those that are primarily components of the cytoplasmic fibrils of the NPC, mislocalize to the cytoplasm. APQ12 deletion also causes defects in NE morphology. In the absence of Apq12, most NPCs appear to be associated with the inner but not the outer nuclear membrane. Low levels of benzyl alcohol, which increases membrane fluidity, prevented Nup mislocalization and restored the proper localization of Nups that had accumulated in cytoplasmic foci upon a shift to lower temperature. Thus, Apq12p connects nuclear pore biogenesis to the dynamics of the NE.

Abbreviations used in this paper: BA, benzyl alcohol; DiOC6, 3,3'- dihexyloxacarbocyanine iodide; IF, immunofluorescence; INM, inner nuclear membrane; NE, nuclear envelope; NPC, nuclear pore complex; Nup, nucleoporin; ONM, outer nuclear membrane; SPB, spindle pole body; UPR, unfolded protein response; UPRE, UPR element; WT, wild type.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

To make a nuclear pore, just relax
Mitch Leslie
J. Cell Biol. 2007 178: 717. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents