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Published online
doi:10.1083/jcb.200702026
The Journal of Cell Biology, Vol. 178, No. 6, 897-904
The Rockefeller University Press, 0021-9525 $30.00
© Salpingidou et al.
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A novel role for the nuclear membrane protein emerin in association of the centrosome to the outer nuclear membrane



Georgia Salpingidou1, Andrei Smertenko1, Irena Hausmanowa-Petrucewicz2, Patrick J. Hussey1, and Chris J. Hutchison1

1 School of Biological and Biomedical Sciences, The University of Durham, Durham DH1 3LE, England, UK
2 Neuromuscular Unit, Medical Research Centre, Polish Academy of Sciences, Warsaw 02-097, Poland

Correspondence to Chris Hutchison: c.j.hutchison{at}durham.ac.uk

The type II inner nuclear membrane protein emerin is a component of the LINC complex that connects the nuclear lamina to the actin cytoskeleton. In emerin-null or -deficient human dermal fibroblasts we find that the centrosome is detached from the nucleus. Moreover, following siRNA knockdown of emerin in wild-type fibroblasts, the centrosome also becomes detached from the nucleus. We show that emerin interacts with tubulin, and that nocadozole-treated wild-type cells phenocopy the detached centrosome characteristic of emerin-null/deficient cells. We also find that a significant fraction of emerin is located at the outer nuclear membrane and peripheral ER, where it interacts directly with the centrosome. Our data provide the first evidence in mammalian cells as to the nature of the linkage of the centrosome, and therefore the tubulin cytoskeleton, with the outer nuclear membrane.

Abbreviations used in this paper: EDMD, Emery Dreifuss muscular dystrophy; HDF, human dermal fibroblast; INM, inner nuclear membrane; LBR, lamin B receptor; MT, microtubule; NE, nuclear envelope; ONM, outer nuclear membrane.


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