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Published online September 17, 2007
doi:10.1083/jcb.200703092
The Journal of Cell Biology, Vol. 178, No. 7, 1251-1264
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Okreglak et al.
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Article

Cofilin recruitment and function during actin-mediated endocytosis dictated by actin nucleotide state



Voytek Okreglak and David G. Drubin

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720

Correspondence to David G. Drubin: drubin{at}berkeley.edu

Cofilin is the major mediator of actin filament turnover in vivo. However, the molecular mechanism of cofilin recruitment to actin networks during dynamic actin-mediated processes in living cells and cofilin's precise in vivo functions have not been determined. In this study, we analyzed the dynamics of fluorescently tagged cofilin and the role of cofilin-mediated actin turnover during endocytosis in Saccharomyces cerevisiae. In living cells, cofilin is not necessary for actin assembly on endocytic membranes but is recruited to molecularly aged adenosine diphosphate actin filaments and is necessary for their rapid disassembly. Defects in cofilin function alter the morphology of actin networks in vivo and reduce the rate of actin flux through actin networks. The consequences of decreasing actin flux are manifested by decreased but not blocked endocytic internalization at the plasma membrane and defects in late steps of membrane trafficking to the vacuole. These results suggest that cofilin-mediated actin filament flux is required for the multiple steps of endocytic trafficking.

Abbreviations used in this paper: CPY, carboxypeptidase Y; F-actin, filamentous actin; lat A, latrunculin A; mRFP, monomeric RFP; SD, synthetic dextrose; TRP, tryptophan; vps, vacuolar protein sorting.


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