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Published online November 12, 2007
doi:10.1083/jcb.200708054
The Journal of Cell Biology, Vol. 179, No. 4, 593-600
The Rockefeller University Press, 0021-9525 $30.00
© 2007 Neumann et al.
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Nuclear size control in fission yeast



Frank R. Neumann and Paul Nurse

The Rockefeller University, New York, NY 10065

Correspondence to Frank Neumann: fneumann{at}rockefeller.edu

A long-standing biological question is how a eukaryotic cell controls the size of its nucleus. We report here that in fission yeast, nuclear size is proportional to cell size over a 35-fold range, and use mutants to show that a 16-fold change in nuclear DNA content does not influence the relative size of the nucleus. Multi-nucleated cells with unevenly distributed nuclei reveal that nuclei surrounded by a greater volume of cytoplasm grow more rapidly. During interphase of the cell cycle nuclear growth is proportional to cell growth, and during mitosis there is a rapid expansion of the nuclear envelope. When the nuclear/cell (N/C) volume ratio is increased by centrifugation or genetic manipulation, nuclear growth is arrested while the cell continues to grow; in contrast, low N/C ratios are rapidly corrected by nuclear growth. We propose that there is a general cellular control linking nuclear growth to cell size.

Abbreviations used in this paper: DIC, differential interference contrast; LMB, leptomycin B; N/C, nuclear/cell; NE, nuclear envelope; ts, temperature sensitive.


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