THE DIFFERENTIATION OF WHITE ADIPOSE CELLS: An Electron Microscope Study

doi:10.1083/jcb.18.3.663

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ARTICLE

THE DIFFERENTIATION OF WHITE ADIPOSE CELLS

: An Electron Microscope Study

Leonard Napolitano Ph.D.¹

¹ From the Department of Anatomy, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania

Differentiating white adipose tissue from presumptive and developingfat pads of newborn and young rats was fixed in buffered osmiumtetroxide, embedded in Vestopal W, and examined in an electronmicroscope. Pre-adipose cells were found to be fibroblasts characterizedby their spindle shape, long tenuous cytoplasmic extensions,and profuse endoplasmic reticulum. The developmental stagestraced from fibroblast to mature adipose cell show a gradualchange in cell shape, an accumulation of cytoplasm and non-membrane-boundedlipid, a decrease in the endoplasmic reticulum, and a changein shape of mitochondria. Transitory glycogen appears at mid-differentiation.Numerous smooth-membraned vesicles occur in the cytoplasm throughoutdifferentiation. Pinocytosis is constantly evident. Cells ofthe multilocular stage are shown to differ from brown fat cells,particularly with respect to cytoplasmic membrane systems andmitochondria. No transport of particulate lipid from the lumenof the capillary to, or within, the adipose cell was detected,nor could any cell organelle be demonstrated to be visibly relatedto lipid synthesis and/or deposition.

Submitted on March 6, 1963

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