JCB logo
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online February 4, 2008
doi:10.1083/jcb.200711136
The Journal of Cell Biology, Vol. 180, No. 3, 473-482
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Fei et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 2268K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fei, W.
Right arrow Articles by Yang, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fei, W.
Right arrow Articles by Yang, H.
Related Collections
Right arrowRelated In this Issue article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Report

Fld1p, a functional homologue of human seipin, regulates the size of lipid droplets in yeast



Weihua Fei1, Guanghou Shui1, Bruno Gaeta2, Ximing Du2, Lars Kuerschner3,4, Peng Li5, Andrew J. Brown2, Markus R. Wenk1, Robert G. Parton3,4, and Hongyuan Yang1,2

1 Department of Biochemistry, National University of Singapore, Singapore 117597, Republic of Singapore
2 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052, New South Wales, Australia
3 Institute for Molecular Bioscience and 4 Centre for Microscopy and Microanalysis, University of Queensland, Brisbane 4072, Australia
5 Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China

Correspondence to Hongyuan Yang: h.rob.yang{at}unsw.edu.au

Lipid droplets (LDs) are emerging cellular organelles that are of crucial importance in cell biology and human diseases. In this study, we present our screen of ~4,700 Saccharomyces cerevisiae mutants for abnormalities in the number and morphology of LDs; we identify 17 fld (few LDs) and 116 mld (many LDs) mutants. One of the fld mutants (fld1) is caused by the deletion of YLR404W, a previously uncharacterized open reading frame. Cells lacking FLD1 contain strikingly enlarged (supersized) LDs, and LDs from fld1{Delta} cells demonstrate significantly enhanced fusion activities both in vivo and in vitro. Interestingly, the expression of human seipin, whose mutant forms are associated with Berardinelli-Seip congenital lipodystrophy and motoneuron disorders, rescues LD-associated defects in fld1{Delta} cells. Lipid profiling reveals alterations in acyl chain compositions of major phospholipids in fld1{Delta} cells. These results suggest that an evolutionally conserved function of seipin in phospholipid metabolism and LD formation may be functionally important in human adipogenesis.

Abbreviations used in this paper: BSCL, Berardinelli-Seip congenital lipodystrophy; CGL, congenital generalized lipodystrophy; LD, lipid droplet; MRM, multiple reaction monitoring; PA, phosphatidic acid; SC, synthetic complete; SE, sterol ester; TAG, triacylglycerol; TEM, transmission EM.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related In this Issue article

Supersized lipid droplets
Mitch Leslie
J. Cell Biol. 2008 180: 445. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents