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Published online
doi:10.1083/jcb.200709019
The Journal of Cell Biology, Vol. 180, No. 6, 1261-1275
The Rockefeller University Press, 0021-9525 $30.00
© Goetz et al.
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Article

Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1



Jacky G. Goetz1,2,3, Bharat Joshi1, Patrick Lajoie1, Scott S. Strugnell1, Trevor Scudamore1, Liliana D. Kojic1, and Ivan R. Nabi1

1 Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3
2 Department of Pathology and Cell Biology, University of Montreal, Montreal, Quebec, Canada H3C 3J7
3 LC1 – UMR CNRS 7175, Institut Gilbert Laustriat Pharmacologie et Physico-chimie, Université Louis Pasteur 1, Faculté de Pharmacie, 67401 Illkirch-Graffenstaden Cedex, France

Correspondence to Ivan R. Nabi: ivan.robert.nabi{at}ubc.ca

Both tyrosine-phosphorylated caveolin-1 (pY14Cav1) and GlcNAc-transferase V (Mgat5) are linked with focal adhesions (FAs); however, their function in this context is unknown. Here, we show that galectin-3 binding to Mgat5-modified N-glycans functions together with pY14Cav1 to stabilize focal adhesion kinase (FAK) within FAs, and thereby promotes FA disassembly and turnover. Expression of the Mgat5/galectin lattice alone induces FAs and cell spreading. However, FAK stabilization in FAs also requires expression of pY14Cav1. In cells lacking the Mgat5/galectin lattice, pY14Cav1 is not sufficient to promote FAK stabilization, FA disassembly, and turnover. In human MDA-435 cancer cells, Cav1 expression, but not mutant Y14FCav1, stabilizes FAK exchange and stimulates de novo FA formation in protrusive cellular regions. Thus, transmembrane crosstalk between the galectin lattice and pY14Cav1 promotes FA turnover by stabilizing FAK within FAs defining previously unknown, interdependent roles for galectin-3 and pY14Cav1 in tumor cell migration.

J.G. Goetz's present address is Integrin Signaling Laboratory, Department of Vascular Biology and Inflammation, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 28029 Madrid, Spain.

Abbreviations used in this paper: Cav1, caveolin-1; FA, focal adhesion; FAK, focal adhesion kinase; FN, fibronectin; Gal-3; galectin-3; Mgat5, GlcNAc-transferase V; p, phospho; SW, swainsonine; Y14, tyrosine-14.


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