Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 2310K) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bloom, O. Articles by Mellman, I. Search for Related Content PubMed PubMed Citation Articles by Bloom, O. Articles by Mellman, I. Pubmed/NCBI databases Gene GEO Profiles HomoloGene Protein UniGene Related Collections Related Article Social Bookmarking                            What's this?

Spinophilin participates in information transfer at immunological synapses

¹ Department of Cell Biology and ² Department of Immunobiology, Ludwig Institute for Cancer Research, and ³ Department of Psychiatry, Yale School of Medicine, New Haven, CT 06520
⁴ Genentech, Inc., South San Francisco, CA 94080

Correspondence to I. Mellman: mellman.ira{at}gene.com

The adaptive immune response is initiated by the presentation of peptides bound to major histocompatibility complex molecules on dendritic cells (DCs) to antigen-specific T lymphocytes at a junction termed the immunological synapse. Although much attention has been paid to cytoplasmic events on the T cell side of the synapse, little is known concerning events on the DC side. We have sought signal transduction components of the neuronal synapse that were also expressed by DCs. One such protein is spinophilin, a scaffolding protein of neuronal dendritic spines that regulates synaptic transmission. In inactive, immature DCs, spinophilin is located throughout the cytoplasm but redistributes to the plasma membrane upon stimulus-induced maturation. In DCs interacting with T cells, spinophilin is polarized dynamically to contact sites in an antigen-dependent manner. It is also required for optimal T cell activation because DCs derived from mice lacking spinophilin exhibit defects in antigen presentation both in vitro and in vivo. Thus, spinophilin may play analogous roles in information transfer at both neuronal and immunological synapses.

J.J. Unternaehrer's present address is Division of Hematology/Oncology, Children's Hospital, Harvard Medical School, Boston, MA 02115.

A. Jiang's present address is Department of Immunobiology, Yale School of Medicine, New Haven, CT 06520.

J.-S. Shin's present address is Department of Immunobiology, University of California, San Francisco, San Francisco, CA 94143.

Abbreviations used in this paper: APC, antigen-presenting cell; BMDC, bone marrow–derived dendritic cell; CFSE, 5(6)-carboxyfluorescein diacetate N-succinimidyl ester; DC, dendritic cell; GPCR, G protein–coupled receptor; IL-2, interleukin-2; IS, immunological synapse; KO, knockout; LPS, lipopolysaccharide; MHCII, major histocompatibility complex type II; PP1, protein phosphatase I; ROI, region of interest; TCR, T cell receptor; WT, wild type.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

Related Article

Spinophilin and the immune synapse

Brian Seed and Ramnik Xavier
J. Cell Biol. 2008 181: 181-183. [Abstract] [Full Text] [PDF]

This article has been cited by other articles:

• Fooksman, D. R., Shaikh, S. R., Boyle, S., Edidin, M. (2009). Cutting Edge: Phosphatidylinositol 4,5-Bisphosphate Concentration at the APC Side of the Immunological Synapse Is Required for Effector T Cell Function. J. Immunol. 182: 5179-5182 [Abstract] [Full Text]  
• Seed, B., Xavier, R. (2008). Spinophilin and the immune synapse. JCB 181: 181-183 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents