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Published online May 26, 2008
doi:10.1083/jcb.200803096
The Journal of Cell Biology, Vol. 181, No. 5, 777-790
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Albertson et al.
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Article

 Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle

Roger Albertson, Jian Cao, Tao-shih Hsieh, and William Sullivan

Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064

Correspondence to William Sullivan: sullivan{at}biology.ucsc.edu

During cytokinesis, cleavage furrow invagination requires an actomyosin-based contractile ring and addition of new membrane. Little is known about how this actin and membrane traffic to the cleavage furrow. We address this through live analysis of fluorescently tagged vesicles in postcellularized Drosophila melanogaster embryos. We find that during cytokinesis, F-actin and membrane are targeted as a unit to invaginating furrows through formation of F-actin–associated vesicles. F-actin puncta strongly colocalize with endosomal, but not Golgi-derived, vesicles. These vesicles are recruited to the cleavage furrow along the central spindle and a distinct population of microtubules (MTs) in contact with the leading furrow edge (furrow MTs). We find that Rho-specific guanine nucleotide exchange factor mutants, pebble (pbl), severely disrupt this F-actin–associated vesicle transport. These transport defects are a consequence of the pbl mutants' inability to properly form furrow MTs and the central spindle. Transport of F-actin–associated vesicles on furrow MTs and the central spindle is thus an important mechanism by which actin and membrane are delivered to the cleavage furrow.

Abbreviations used in this paper: Dlg, discs large; MT, microtubule; NRK, normal rat kidney; n-Syb, n-synaptobrevin; RE, recycling endosomes; Syt, synaptotagmin; WT, wild type.

© 2008 Albertson et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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