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Published online June 9, 2008
doi:10.1083/jcb.200803042
The Journal of Cell Biology, Vol. 181, No. 6, 885-892
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Jaco et al.
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Centromere mitotic recombination in mammalian cells



Isabel Jaco, Andrés Canela, Elsa Vera, and Maria A. Blasco

Telomeres and Telomerase Group, Molecular Oncology Program, Spanish National Cancer Centre, 28029 Madrid, Spain

Correspondence to Maria A. Blasco: mblasco{at}cnio.es

Centromeres are special structures of eukaryotic chromosomes that hold sister chromatid together and ensure proper chromosome segregation during cell division. Centromeres consist of repeated sequences, which have hindered the study of centromere mitotic recombination and its consequences for centromeric function. We use a chromosome orientation fluorescence in situ hybridization technique to visualize and quantify recombination events at mouse centromeres. We show that centromere mitotic recombination occurs in normal cells to a higher frequency than telomere recombination and to a much higher frequency than chromosome-arm recombination. Furthermore, we show that centromere mitotic recombination is increased in cells lacking the Dnmt3a and Dnmt3b DNA methyltransferases, suggesting that the epigenetic state of centromeric heterochromatin controls recombination events at these regions. Increased centromere recombination in Dnmt3a,3b-deficient cells is accompanied by changes in the length of centromere repeats, suggesting that prevention of illicit centromere recombination is important to maintain centromere integrity in the mouse.

Abbreviations used in this paper: CENP-B, centromere protein B; CO-FISH, chromosome orientation FISH; C-SCE, centromere sister chromatid exchange; ES, embryonic stem; MEF, mouse embryonic fibroblast; PNA, peptide nucleic acid; Q-FISH, quantitative FISH.

© 2008 Jaco et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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Centromeres cross over, a lot
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