JCB logo
PeproTech: Your source for Cell Biology Research Reagents
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online June 23, 2008
doi:10.1083/jcb.200712137
The Journal of Cell Biology, Vol. 181, No. 7, 1047-1054
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Goss et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 2865K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Goss, J. W.
Right arrow Articles by Toomre, D. K.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goss, J. W.
Right arrow Articles by Toomre, D. K.
Related Collections
Right arrowRelated In this Issue article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Report

 Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis

John W. Goss and Derek K. Toomre

Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510

Correspondence to Derek K. Toomre: derek.toomre{at}yale.edu

Membrane trafficking during cytokinesis is not well understood. We used advanced live cell imaging techniques to track exocytosis of single vesicles to determine whether constitutively exocytosed membrane is focally delivered to the cleavage furrow. Ultrasensitive three-dimensional confocal time-lapse imaging of the temperature-sensitive membrane cargo protein vesicular stomatitis virus protein–yellow fluorescent protein revealed that vesicles from both daughter cells traffic out of the Golgi and into the furrow, following curvilinear paths. Immunolocalization and photobleaching experiments indicate that individual vesicles accumulate at the midbody and generate a reserve vesicle pool that is distinct from endosomal and lysosomal compartments. Total internal reflection fluorescence microscopy imaging provided direct evidence that Golgi-derived vesicles from both daughter cells not only traffic to the furrow region but dock and fuse there, supporting a symmetrically polarized exocytic delivery model. In contrast, quantitative analysis of midbody abscission showed inheritance of the midbody remnant by one daughter cell, indicating that cytokinesis is composed of both symmetrical and asymmetrical stages.

Abbreviations used in this paper: DIC, differential interference contrast; EMMA, exocytic midzone membrane accumulation; GPI, glycosylphosphatidylinositol; ROI, region of interest; SDCM, spinning disc confocal microscopy; TGN, trans-Golgi network; TIRFM, total internal reflection fluorescence microscopy; VSVG, vesicular stomatitis virus glycoprotein.

© 2008 Goss and Toomre This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related In this Issue article

Daughter cells share duties
Mitch Leslie
J. Cell Biol. 2008 181: 1043. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents