JCB logo
IN Cell Analyzer 2000
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online
doi:10.1083/jcb.200712080
The Journal of Cell Biology, Vol. 182, No. 1, 27-33
The Rockefeller University Press, 0021-9525 $30.00
© Tan et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 1823K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tan, S.
Right arrow Articles by Bennett, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tan, S.
Right arrow Articles by Bennett, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Report

Mars promotes dTACC dephosphorylation on mitotic spindles to ensure spindle stability



Shengjiang Tan1,2, Ekaterina Lyulcheva1,2, Jon Dean1, and Daimark Bennett1,2

1 Department of Zoology, Oxford University, Oxford OX1 3PS, England, UK
2 School of Biological Sciences, Biosciences Building, University of Liverpool, Liverpool L69 7ZB, England, UK

Correspondence to Daimark Bennett: daimark.bennett{at}liverpool.ac.uk

Microtubule-associated proteins (MAPs) ensure the fidelity of chromosome segregation by controlling microtubule (MT) dynamics and mitotic spindle stability. However, many aspects of MAP function and regulation are poorly understood in a developmental context. We show that mars, which encodes a Drosophila melanogaster member of the hepatoma up-regulated protein family of MAPs, is essential for MT stabilization during early embryogenesis. As well as associating with spindle MTs in vivo, Mars binds directly to protein phosphatase 1 (PP1) and coimmunoprecipitates from embryo extracts with minispindles and Drosophila transforming acidic coiled-coil (dTACC), two MAPs that function as spindle assembly factors. Disruption of binding to PP1 or loss of mars function results in elevated levels of phosphorylated dTACC on spindles. A nonphosphorylatable form of dTACC is capable of rescuing the lethality of mars mutants. We propose that Mars mediates spatially controlled dephosphorylation of dTACC, which is critical for spindle stabilization.

Abbreviations used in this paper: dTACC, Drosophila transforming acidic coiled coil; FM, FLAG-Myc; HURP, hepatoma up-regulated protein; MAP, microtubule-associated protein; Msps, minispindles; MT, microtubule; p-dTACC; phosphorylated dTACC; PP1, protein phosphatase 1.

© 2008 Tan et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents