JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online
doi:10.1083/jcb.200802053
The Journal of Cell Biology, Vol. 182, No. 2, 277-288
The Rockefeller University Press, 0021-9525 $30.00
© Yamamoto et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 2336K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamamoto, A.
Right arrow Articles by Hiraoka, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamamoto, A.
Right arrow Articles by Hiraoka, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Article

Spindle checkpoint activation at meiosis I advances anaphase II onset via meiosis-specific APC/C regulation



Ayumu Yamamoto1,2, Kenji Kitamura3, Daisuke Hihara1, Yukinobu Hirose1, Satoshi Katsuyama1, and Yasushi Hiraoka2

1 Department of Chemistry, Shizuoka University, Suruga-ku, Shizuoka 422-8529, Japan
2 Cell Biology Group, Kansai Advanced Research Center, National Institute of Information and Communications Technology, Nishi-ku, Kobe 651-2492, Japan
3 Center for Gene Science, Hiroshima University, Higashi Hiroshima 739-8527, Japan

Correspondence to Ayuma Yamamoto: sayamam{at}ipc.shizuoka.ac.jp

During mitosis, the spindle assembly checkpoint (SAC) inhibits the Cdc20-activated anaphase-promoting complex/cyclosome (APC/CCdc20), which promotes protein degradation, and delays anaphase onset to ensure accurate chromosome segregation. However, the SAC function in meiotic anaphase regulation is poorly understood. Here, we examined the SAC function in fission yeast meiosis. As in mitosis, a SAC factor, Mad2, delayed anaphase onset via Slp1 (fission yeast Cdc20) when chromosomes attach to the spindle improperly. However, when the SAC delayed anaphase I, the interval between meiosis I and II shortened. Furthermore, anaphase onset was advanced and the SAC effect was reduced at meiosis II. The advancement of anaphase onset depended on a meiosis-specific, Cdc20-related factor, Fzr1/Mfr1, which contributed to anaphase cyclin decline and anaphase onset and was inefficiently inhibited by the SAC. Our findings show that impacts of SAC activation are not confined to a single division at meiosis due to meiosis-specific APC/C regulation, which has probably been evolved for execution of two meiotic divisions.

Abbreviations used in this paper: APC/C, anaphase promoting complex/cyclosome; MI, meiosis I; MII, meiosis II; SAC, spindle assembly checkpoint; SPB, spindle pole body.

© 2008 Yamamoto et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents