JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online July 28, 2008
doi:10.1083/jcb.200801027
The Journal of Cell Biology, Vol. 182, No. 2, 341-353
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Kueh et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 4594K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kueh, H. Y.
Right arrow Articles by Brieher, W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kueh, H. Y.
Right arrow Articles by Brieher, W. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Article

Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers



Hao Yuan Kueh1,2, Guillaume T. Charras3,4, Timothy J. Mitchison1, and William M. Brieher1,5

1 Department of Systems Biology, Harvard Medical School, Boston, MA 02115
2 Graduate Program in Biophysics, Harvard University, Cambridge, MA 02138
3 London Centre for Nanotechnology and 4 Department of Cell and Developmental Biology, Faculty of Life Sciences, University College London, London, England, UK
5 Department of Cell and Developmental Biology, University of Illinois, Urbana, IL 61801

Correspondence to William M. Brieher: wbrieher{at}uiuc.edu

Turnover of actin filaments in cells requires rapid actin disassembly in a cytoplasmic environment that thermodynamically favors assembly because of high concentrations of polymerizable monomers. We here image the disassembly of single actin filaments by cofilin, coronin, and actin-interacting protein 1, a purified protein system that reconstitutes rapid, monomer-insensitive disassembly (Brieher, W.M., H.Y. Kueh, B.A. Ballif, and T.J. Mitchison. 2006. J. Cell Biol. 175:315–324). In this three-component system, filaments disassemble in abrupt bursts that initiate preferentially, but not exclusively, from both filament ends. Bursting disassembly generates unstable reaction intermediates with lowered affinity for CapZ at barbed ends. CapZ and cytochalasin D (CytoD), a barbed-end capping drug, strongly inhibit bursting disassembly. CytoD also inhibits actin disassembly in mammalian cells, whereas latrunculin B, a monomer sequestering drug, does not. We propose that bursts of disassembly arise from cooperative separation of the two filament strands near an end. The differential effects of drugs in cells argue for physiological relevance of this new disassembly pathway and potentially explain discordant results previously found with these drugs.

Abbreviations used in this paper: Aip1, actin-interacting protein 1; CCD, charge-coupled device; CytoD, cytochalasin D; df, degrees of freedom; KabC, kabiramide C; LatB, latrunculin B; PAGFP, photoactivatable GFP; mRFP, monomeric red fluorescence protein.

© 2008 Kueh et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents