Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 1698K) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Espenel, C. Articles by Milhiet, P.-E. Search for Related Content PubMed PubMed Citation Articles by Espenel, C. Articles by Milhiet, P.-E. Social Bookmarking                            What's this?

Single-molecule analysis of CD9 dynamics and partitioning reveals multiple modes of interaction in the tetraspanin web

¹ Institut National de la Santé et de la Recherche Medicale, Unité 554, 34090 Montpellier, France
² Université de Montpellier, Centre National de la Recherche Scientifique, Unité Mixte Recherche 5048, Centre de Biochimie Structurale, 34090 Montpellier, France
³ Institut National de la Santé et de la Recherche Medicale, Unité 602,94804 Villejuif, France
⁴ Université Paris 11, Institut André Lwoff, 94801 Villejuif, France

Correspondence to Pierre-Emmanuel Milhiet: pem{at}cbs.cnrs.fr

Tetraspanins regulate cell migration, sperm–egg fusion, and viral infection. Through interactions with one another and other cell surface proteins, tetraspanins form a network of molecular interactions called the tetraspanin web. In this study, we use single-molecule fluorescence microscopy to dissect dynamics and partitioning of the tetraspanin CD9. We show that lateral mobility of CD9 in the plasma membrane is regulated by at least two modes of interaction that each exhibit specific dynamics. The majority of CD9 molecules display Brownian behavior but can be transiently confined to an interaction platform that is in permanent exchange with the rest of the membrane. These platforms, which are enriched in CD9 and its binding partners, are constant in shape and localization. Two CD9 molecules undergoing Brownian trajectories can also codiffuse, revealing extra platform interactions. CD9 mobility and partitioning are both dependent on its palmitoylation and plasma membrane cholesterol. Our data show the high dynamic of interactions in the tetraspanin web and further indicate that the tetraspanin web is distinct from raft microdomains.

© 2008 Espenel et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Charrin, S., Yalaoui, S., Bartosch, B., Cocquerel, L., Franetich, J.-F., Boucheix, C., Mazier, D., Rubinstein, E., Silvie, O. (2009). The Ig Domain Protein CD9P-1 Down-regulates CD81 Ability to Support Plasmodium yoelii Infection. J. Biol. Chem. 284: 31572-31578 [Abstract] [Full Text]  
• Weng, J., Krementsov, D. N., Khurana, S., Roy, N. H., Thali, M. (2009). Formation of Syncytia Is Repressed by Tetraspanins in Human Immunodeficiency Virus Type 1-Producing Cells. J. Virol. 83: 7467-7474 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents