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Published online
doi:10.1083/jcb.200802085
The Journal of Cell Biology, Vol. 182, No. 6, 1099-1111
The Rockefeller University Press, 0021-9525 $30.00
© Jwa et al.
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Article

Regulation of Sli15/INCENP, kinetochore, and Cdc14 phosphatase functions by the ribosome biogenesis protein Utp7



Miri Jwa1, Jae-hyun Kim2, and Clarence S.M. Chan1,2

1 Institute for Cellular and Molecular Biology and 2 Section of Molecular Genetics and Microbiology, The University of Texas, Austin, TX 78712

Correspondence to Clarence S.M. Chan: clarence_chan{at}mail.utexas.edu

The Sli15–Ipl1–Bir1 chromosomal passenger complex is essential for proper kinetochore–microtubule attachment and spindle stability in the budding yeast Saccharomyces cerevisiae. During early anaphase, release of the Cdc14 protein phosphatase from the nucleolus leads to the dephosphorylation of Sli15 and redistribution of this complex from kinetochores to the spindle. We show here that the predominantly nucleolar ribosome biogenesis protein Utp7 is also present at kinetochores and is required for normal organization of kinetochore proteins and proper chromosome segregation. Utp7 associates with and regulates the localization of Sli15 and Cdc14. Before anaphase onset, it prevents the premature nucleolar release of Cdc14 and the premature concentration of Sli15 on the spindle. Furthermore, Utp7 can regulate the localization and phosphorylation status of Sli15 independent of its effect on Cdc14 function. Thus, Utp7 is a multifunctional protein that plays essential roles in the vital cellular processes of ribosome biogenesis, chromosome segregation, and cell cycle control.

Abbreviations used in this paper: ChIP, chromatin immunoprecipitation; HU, hydroxyurea; MEN, mitotic exit network; rRNA, ribosomal RNA; SSU, small subunit.

© 2008 Jwa et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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