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Published online
doi:10.1083/jcb.200801038
The Journal of Cell Biology, Vol. 183, No. 1, 29-36
The Rockefeller University Press, 0021-9525 $30.00
© O'Connell et al.
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The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes



Christopher B. O'Connell1, Jadranka Loncarek1, Polla Hergert1, Antonis Kourtidis2, Douglas S. Conklin2, and Alexey Khodjakov1

1 Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, NY 12201
2 GenNYSis Center for Excellence in Cancer Genomics, University at Albany, Rensselaer, NY 12144

Correspondence to Christopher B. O'Connell: oconnell{at}wadsworth.org

The accuracy of chromosome segregation is enhanced by the spindle assembly checkpoint (SAC). The SAC is thought to monitor two distinct events: attachment of kinetochores to microtubules and the stretch of the centromere between the sister kinetochores that arises only when the chromosome becomes properly bioriented. We examined human cells undergoing mitosis with unreplicated genomes (MUG). Kinetochores in these cells are not paired, which implies that the centromere cannot be stretched; however, cells progress through mitosis. A SAC is present during MUG as cells arrest in response to nocodazole, taxol, or monastrol treatments. Mad2 is recruited to unattached MUG kinetochores and released upon their attachment. In contrast, BubR1 remains on attached kinetochores and exhibits a level of phosphorylation consistent with the inability of MUG spindles to establish normal levels of centromere tension. Thus, kinetochore attachment to microtubules is sufficient to satisfy the SAC even in the absence of interkinetochore tension.

Abbreviations used in this paper: CENP-A, centromere protein A; HU, hydroxyurea; INCENP, inner centromere protein; MUG, mitosis with unreplicated genomes; SAC, spindle assembly checkpoint.

© 2008 O'Connell et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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J. Cell Biol. 2008 183: 2. [Full Text] [PDF]



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